Effect and mechanism of reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis

Figure 3 Reactive oxygen species-mediated NOD-like receptor family pyrin domain-containing 3 inflammasome activation in hepatic alveolar echinococcosis rats. A: Reactive oxygen species (ROS) production in the 20, 50, and 100 mg/kg/d N-tert-Butyl-α-phenylnitrone (PBN) groups; B: ROS production in the 50 mg/kg/d PBN, normal saline (NS), and control groups; C: Analysis of inflammation; D: Immunohistochemical staining of NLRP3, caspase-1, interleukin (IL)-1β, and IL-18; E: Relative expression of NLRP3, caspase-1, IL-1β, and IL-18 in the marginal zone compared with the corresponding normal liver; F: Western blotting analysis of the PBN, NS, and control groups; G: NLRP3 IntDen/β-actin IntDen protein expression; H: Caspase-1 IntDen/β-actin IntDen protein expression; I: Relationship between ROS production and the relative expression of NLRP3; J: The relative expression of NLRP3 and inflammation observed in the marginal zone. Western blotting was performed in triplicate (mean ± SD). Scale bar, 50 μm. A, n = 3 rats per group; B, C, and E, n = 10 rats per group; I and J, n = 10. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001; ^dP < 0.0001. NLRP3: NOD-like receptor family pyrin domain-containing 3; ROS: Reactive oxygen species; PBN: N-tert-Butyl-α-phenylnitrone; NS: Normal saline; IOD: Integral optical density; IL-1β: Interleukin-1β; IL-18: Interleukin-18.