Zhang L, Lee H, Grimm MC, Riordan SM, Day AS, Lemberg DA. Campylobacter concisus and inflammatory bowel disease. World J Gastroenterol 2014; 20(5): 1259-1267 [PMID: 24574800 DOI: 10.3748/wjg.v20.i5.1259]
Corresponding Author of This Article
Li Zhang, MBBS, PhD, Senior Lecturer (Medical Microbiology and Immunology), School of Biotechnology and Biomolecular Sciences, University of New South Wales, High St, Kensington, Sydney, NSW 2052, Australia. l.zhang@unsw.edu.au
Research Domain of This Article
Microbiology
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Li Zhang, Hoyul Lee, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia
Michael C Grimm, St George and Sutherland Clinical School, University of New South Wales, Sydney, NSW 2052, Australia
Stephen M Riordan, Gastrointestinal and Liver Unit, The Prince of Wales Hospital, Prince of Wales Clinical School, University of New South Wales, Sydney, NSW 2052, Australia
Andrew S Day, Daniel A Lemberg, Department of Gastroenterology, Sydney Children’s Hospital, Sydney, NSW 2031, Australia
Andrew S Day, Department of Pediatrics, University of Otago, Christchurch, North Dunedin 9016, New Zealand
Andrew S Day, Daniel A Lemberg, School of Women’s and Children’s Health, University of New South Wales, Sydney, NSW 2031, Australia
ORCID number: $[AuthorORCIDs]
Author contributions: Zhang L wrote the review and identified the motifs; Lee H analyzed genes and proteins in putative prophages; Grimm MC, Riordan SM, Day AS and Lemberg DA provided critical feedback and helped in editing the manuscript.
Correspondence to: Li Zhang, MBBS, PhD, Senior Lecturer (Medical Microbiology and Immunology), School of Biotechnology and Biomolecular Sciences, University of New South Wales, High St, Kensington, Sydney, NSW 2052, Australia. l.zhang@unsw.edu.au
Telephone: +61-1-93852042 Fax: +61-2-93851483
Received: September 27, 2013 Revised: November 7, 2013 Accepted: December 12, 2013 Published online: February 7, 2014 Processing time: 145 Days and 20.5 Hours
Abstract
Investigation of the possible role of Campylobacter concisus (C. concisus) in inflammatory bowel disease (IBD) is an emerging research area. Despite the association found between C. concisus and IBD, it has been difficult to explain how C. concisus, a bacterium that is commonly present in the human oral cavity, may contribute to the development of enteric diseases. The evidence presented in this review shows that some C. concisus strains in the oral cavity acquired zonula occludens toxin (zot) gene from a virus (prophage) and that C. concisus Zot shares conserved motifs with both Vibrio cholerae Zot receptor binding domain and human zonulin receptor binding domain. Both Vibrio cholerae Zot and human zonulin are known to increase intestinal permeability by affecting the tight junctions. Increased intestinal permeability is a feature of IBD. Based on these data, we propose that a primary barrier function defect caused by C. concisus Zot is a mechanism by which zot-positive C. concisus strains may trigger the onset and relapse of IBD.
Core tip:Campylobacter concisus (C. concisus) is an oral bacterium that was previously shown to be associated with inflammatory bowel disease (IBD). Evidence presented in this review shows that some strains of C. concisus acquired zonula occludens toxin (zot) gene from a virus (prophage), suggesting that a primary barrier function defect caused by C. concisus Zot is a mechanism by which zot-positive C. concisus strains may trigger the onset and relapse of IBD.
Citation: Zhang L, Lee H, Grimm MC, Riordan SM, Day AS, Lemberg DA. Campylobacter concisus and inflammatory bowel disease. World J Gastroenterol 2014; 20(5): 1259-1267
Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract[1]. The two major clinical forms of IBD are Crohn’s disease (CD) and ulcerative colitis (UC). The etiology of IBD is not fully understood. Multiple contributors including genetic factors, environmental factors and intestinal microbiota have been suggested to play a role in the development of IBD[1]. The pathogenesis of IBD is thought to result from a dysregulated response of the intestinal mucosal immune system to luminal commensal microbes[1].
The highest incidence of IBD, both CD and UC, is in young adults[2]. This implies that in most of the patients with IBD, the intestinal mucosal immune system has maintained a non-hostile relationship with the intestinal commensal microbes for decades prior to the onset of the disease. Given this, the uncontrolled attack of the intestinal mucosal immune system to luminal commensal bacteria would have been initiated by a trigger. Such a trigger may not necessarily be a dominant intestinal bacterial species or a long-term intestinal resident microbe. Evidence presented in this review suggests that zonula occludens toxin gene (zot)-positive Campylobacter concisus (C. concisus) strains, a bacterium colonizing the human oral cavity, may be a trigger of IBD.
C. concisus
C. concisus is a Gram negative bacterium that requires microaerobic or anaerobic conditions enriched with H2 for growth[3]. Cells of C. concisus are curved, with a size of (0.5 - 1) × (2 - 6) μm. C. concisus is motile, driven by a single polarized flagellum (Figure 1).
Figure 1 Electron microscopic image of Campylobacter concisus.
HUMANS ARE THE NATURAL HOST OF C. CONCISUS AND THE ORAL CAVITY IS THE PRIMARY COLONIZATION SITE
Tanner et al[4] first reported the isolation of C. concisus from patients with gingivitis in 1981. Zhang et al[5] examined the presence of C. concisus in saliva samples obtained from healthy individuals of different age groups and found that C. concisus is commonly present in the human oral cavity. In that study, C. concisus was detected in 97% (57/59) of saliva samples collected from healthy individuals aged 3-60 years old by polymerase chain reaction (PCR) targeting 16S rRNA gene and cultured from 75% (44/59) of these saliva samples using a filtration method. A study from Petersen et al[6] also detected a high prevalence of C. concisus in the human oral cavity: in this study C. concisus was detected in 100% of saliva samples (11/11) collected from healthy individuals by a PCR targeting 16S rRNA gene. Despite its high prevalence in the human oral cavity, C. concisus is not a dominant oral bacterial species[7].
In comparison to the high isolation of C. concisus from saliva samples, the isolation rates of C. concisus from fecal samples collected from healthy individuals were much lower. Using the filtration method, Engberg et al[8] isolated C. concisus from 2.8% (3/107) of fecal samples and Nielsen et al[9] did not isolate C. concisus from any of 108 fecal samples collected from healthy individuals. The low isolation rates of C. concisus from fecal samples suggest that the human intestinal tract of healthy individuals is a less optimal site for C. concisus colonization compared to the oral cavity.
To date, C. concisus has not been detected in any healthy animals. In a study examining the presence of Campylobacter species in fecal samples collected from 70 healthy pet dogs using a quantitative PCR targeting the 60 kDa chaperonin gene, Chaban et al[10] detected seven different campylobacter species but not C. concisus. Various campylobacter species have been detected in fecal samples collected from animals or birds, but C. concisus was not detected[3,10,11]. Lynch et al[12] isolated C. concisus from 10% (18/185) of chicken meat and 3% of beef meat (6/186) samples. However, whether chicken and cattle are natural hosts of C. concisus cannot be determined from these data.
C. concisus has been detected in some animals with gastrointestinal disorders. Petersen et al[6] detected C. concisus in 12.5% (1/8) of saliva samples from pet cats with dental diseases by PCR targeting the 16S rRNA gene[6]. In addition, Chaban et al[10] detected C. concisus in fecal samples of 9% of dogs with diarrhea (6/65).
The collective data suggest that humans are the natural host of C. concisus, with the human oral cavity being the primary colonization site (Table 1).
Table 1 Detection of Campylobacter concisus in samples obtained from healthy individuals.
Samples
Cultivation of C. concisus
Detection of C. concisus DNA by PCR
Human saliva
75%
97%-100%
Human feces
0%-2.8%
33%
Human intestinal biopsies
0%
2%-38%
DIVERSITY OF C. CONCISUS STRAINS COLONIZING THE HUMAN ORAL CAVITY
C. concisus strains colonizing the human oral cavity are greatly diverse. On examination of oral C. concisus strains isolated from individual patients with IBD and healthy controls, it was found that C. concisus strains isolated from each individual had unique protein patterns on sodium dodecyl sulphate polyacrylamide gel electrophoresis[5,13,14]. Furthermore, some individuals were colonized with multiple C. concisus strains in the oral cavity, with as many as three different C. concisus strains having been isolated from individual patients with IBD or healthy controls[5,13,14]. A significantly higher number of patients with active IBD were colonized with multiple C. concisus strains in the oral cavity compared to healthy controls14].
COLONIZATION OF ORAL C. CONCISUS STRAINS IN THE INTESTINAL TRACT
It is estimated that 1-1.5 L of saliva is produced daily in humans, most of which is swallowed[15]. Thus, the human oral cavity is a constantly available source, transporting C. concisus from the oral cavity along with saliva to lower parts of the gastrointestinal tract. However, as mentioned previously, both the detection of C. concisus by PCR and the cultivation of C. concisus from fecal samples were much lower than that from saliva samples, indicating that C. concisus routinely transported from the oral cavity to the intestines does not commonly establish colonization there.
Nevertheless, intestinal colonization of oral C. concisus strains does occur in some individuals. For example, in a study comparing the housekeeping genes of C. concisus strains isolated from intestinal biopsies of patients with IBD and controls, Ismail et al[13] found that a C. concisus strain isolated from intestinal biopsies of a patient with UC had housekeeping genes identical to that of an oral C. concisus strain isolated from the same patient, providing evidence that the oral C. concisus strains are in fact able to colonize the human intestinal tract (Table 2).
Table 2 Genetic relatedness of enteric and oral Campylobacter concisus strains.
Enteric C. concisus strains from patients with IBD
Genetically related C. concisus strains
A strain isolated from intestinal biopsies of patient No. 1
An oral strain from patient No. 6
A strain isolated from intestinal biopsies of patient No. 3
An oral strain from patient No. 3 (identical)
A strain isolated from luminal fluid of patient No. 3
An oral strain from healthy No. 1
In addition to an individual’s own oral C. concisus, C. concisus detected in the intestinal tract may also come from a different source, most likely through materials contaminated with saliva from others. For example, in the above patient with UC, while the C. concisus strain isolated from intestinal biopsies had housekeeping genes identical to that of the patient’s own oral C. concisus strain, the C. concisus strain isolated from the luminal fluid of this patient had a genetic relationship closely related to an oral C. concisus strain from a healthy control, rather than to the patient’s own oral C. concisus (Table 2)[13].
The human intestinal environment is not optimal for C. concisus colonization in general, as suggested by the low isolation rate of C. concisus from fecal samples of healthy individuals (Table 1). Given this, C. concisus intestinal colonization is most likely a short-term event in most individuals. However, with the human oral cavity as a constantly available source of C. concisus, repeated intestinal colonization of C. concisus may occur.
Whether the characteristics of a given oral C. concisus strain or the intestinal environment of an individual, or both, plays the major role in determining whether or not intestinal colonization of oral C. concisus strains will occur is currently unknown. A previous study by Haag et al[16] showed that intestinal microbiota shifts towards elevated commensal Escherichia coli loads abrogated colonization resistance against Campylobacter jejuni in mice. Currently, it is not clear whether the dysbiosis associated with IBD plays a role in C. concisus intestinal colonization.
PREVALENCE OF C. CONCISUS IN THE INTESTINAL TRACT OF PATIENTS WITH IBD AND HEALTHY CONTROLS
A number of studies have examined the prevalence of C. concisus in the intestinal tract of patients with IBD using PCR methods. Most of these studies detected a significantly higher prevalence of C. concisus DNA in patients with IBD and controls[17-21]. The reported detection rates of C. concisus by PCR in enteric samples (biopsies and fecal samples) were 33%-69% in patients with IBD and 2%-38% in controls[17-21]. Analysis of the data in these studies revealed a number of interesting findings.
Firstly, different PCR strategies affect the detection of C. concisus in enteric samples. This was best seen in the study conducted by Man et al[18], who compared the prevalence of C. concisus in fecal samples collected from 54 children with CD, 33 healthy controls and 27 non-IBD controls using two different PCR methods. The first PCR method employed campylobacter genus specific PCR (primers C412F/C1288R) and sequencing the PCR products to determine campylobacter species. The second PCR method was a nested PCR, using campylobacter genus specific PCR (primers C412F/C1288R) followed by C. concisus specific PCR (primers Concisus F/Concisus R). These two PCR methods yielded very different results in detection of C. concisus in the same samples. The prevalence of C. concisus in children with CD, healthy controls and non-IBD controls detected by C. concisus genus specific PCR was 19% (10/54), 12% (4/33) and zero (0/27) respectively. The nested PCR greatly increased the detection of C. concisus in the same cohort of samples, with the prevalence of C. concisus being 65% (35/54) in children with CD, 33% (11/33) in healthy controls and 37% (10/27) in non-IBD controls. The nested PCR, but not the genus specific PCR, detected a significantly higher prevalence of C. concisus in children with CD as compared to healthy controls[18] (Table 3). Indeed, in studies revealing a significant difference in intestinal prevalence of C. concisus between patients with IBD and controls, nested PCR was used to examine all of the samples or part of the samples[17-20].
Table 3 Detection rates of Campylobacter concisus in fecal samples by two polymerase chain reaction methods.
Campylobacter genus PCR
Nested PCR
CD (n = 54)
19%
65%
Healthy controls (n = 33)
12%
33%
Non-IBD controls (n = 27)
0
37%
Secondly, collection of multiple intestinal biopsies increases the detection of intestinal prevalence of C. concisus. A study by Mahendran et al[20] showed that in comparison to the collection of one biopsy, the collection of four biopsies from each individual greatly increased the detection of C. concisus (Figure 2).
Figure 2 An example showing that collection of multiple intestinal biopsies increases the detection of intestinal prevalence of Campylobacter concisus.
Data included in Figure 2 were from reference 20. In both patients with inflammatory bowel disease (IBD) (right column) and healthy controls (left column), the detection of intestinal prevalence of Campylobacter concisus (C. concisus) was greatly increased using four biopsies as compared to using one biopsy. In healthy controls, C. concisus detection rates in a single biopsy collected from ileum, caecum, colon and rectum were 21%, 18%, 18% and 9% respectively. If results from the four biopsies were used in determining the intestinal prevalence of C. concisus, the intestinal prevalence of C. concisus in healthy controls was 36%. Similarly, in patients with IBD, C. concisus detection rates in a single biopsy collected from ileum, caecum, colon and rectum were 23%, 15%, 43% and 26% respectively, and the intestinal prevalence of C. concisus in patients with IBD was 68% when four biopsies were taken into consideration.
Thirdly, despite the increased prevalence of C. concisus detected by PCR in the intestinal tract of patients with IBD, the isolation rates of C. concisus from intestinal biopsies of patients with IBD were low (3%-7.7%)[17,20,21]. This suggests that C. concisus detected in most of the enteric samples were at a low number or in a nonculturable state.
ADVERSE EFFECTS OF C. CONCISUS ON INTESTINAL EPITHELIAL CELLS
A number of adverse effects of C. concisus on intestinal epithelial cells have been described. Using in vitro cell culture models (Caco2 cells or HT-29 cells), epithelial adhesion and invasion, damage of the barrier function and up-regulation of Toll-like receptors-4 expression by C. concisus have been reported. Different strains showed varying degrees of ability to induce such adverse effects[13,22-25]. The underlying molecular mechanisms responsible for these effects have not yet been investigated.
C. CONCISUS ZOT GENE AND IBD
Mahendran et al[14] examined the prevalence of zot gene in 56 oral C. concisus strains isolated from saliva of 19 patients with IBD and 20 healthy controls. This study showed that 30% (17/56) of the oral C. concisus strains carried the zot gene. The zot-positive C. concisus strain was present in 55% (6/11) of patients with active IBD and 40% (8/20) of healthy controls. Some IBD patients with active disease 18% (2/11) were colonized with multiple zot-positive C. concisus strains in the oral cavity. Interestingly, polymorphic forms of the C. concisus zot gene resulting in the substitution of valine at amino acid position 270 were found to be associated with active IBD.
The zot gene was first discovered in Vibrio cholerae where it is carried by a filamentous prophage[26,27]. The zot gene in V. cholerae is required for phage production; a V. cholerae strain with zot gene mutation did not produce phage particles into the culture supernatant[28]. The V. cholerae Zot toxin was shown to increase intestinal permeability and to be associated with mild to moderate diarrhea[26,29,30].
Previous studies have found a human intestinal Zot analogue, namely zonulin, which is a physiological regulator that increases the intestinal permeability[31,32].
C. CONCISUS ZOT GENE IS A COMPONENT OF A CHROMOSOMALLY INTEGRATED PUTATIVE PROPHAGE
Stothard et al[33] established a visual database of bacterial chromosome maps in which C. concisus strain 13826 (Accession No. CP000792. 1) was included. Stothard et al[33] identified a region from nucleotide position 1576683 to 1615449 (38767 bp) in the genome of C. concisus strain 13826 as an “incomplete prophage”. In this region, 39 genes with open reading frames were identified, with four of these genes encoding integrases and 10 genes encoding phage-like proteins[33].
The genetic structures of this “incomplete prophage” region are shown in Figure 3A and the proteins encoded by genes in this region are shown in Table 4. We compared the genes within this region using publicly available softwares[34,35]. A number of genes that have identical nucleotide sequences, including CCC13826_1099, CCC13826_0020, CCC13826_1102, CCC13826_0164, CCC13826_0185, CCC13826_2082 and CCC13826_2077 were annotated with identical protein names.
Figure 3 Genetic structures of putative prophage identified in Campylobacter concisus strain 13826.
A: Multiple prophages at nucleotide position between 1576686 and 1614075; B: One putative prophage at nucleotide position between 939158 and 946901. Identical genes were indicated with the same color. int: A gene encodes phage integrase; zot: Zonula occludens toxin gene. The numbers are the locus tags.
Table 4 Proteins encoded by putative prophages in Campylobacter concisus strain 13826.
Nucleotide position
Locus tag
Encoded proteins
Size (aa)
1576686..1581986
CCC13826_0568
Hypothetical protein
1766
1582376..1583269
CCC13826_0638
Phage integrase
297
1583269..1583880
CCC13826_2272
Glutathionylspermidine synthase family
203
1583895..1585235
CCC13826_2273
Bacteriophage replication gene A protein
446
1585467..1585853
CCC13826_2274
Protein yitk
128
1585894..1586115
CCC13826_1101
Phosphonate uptake transporter
73
1586807..1587043
CCC13826_1102
Sensory box protein
78
1587044..1587496
CCC13826_2275
Hypothetical protein
150
1587598..1588506
CCC13826_2082
Phage integrase
302
1588755..1589966
CCC13826_1099
Putative phage replication protein A
403
1590015..1591184
CCC13826_1100
ATP binding protein (ABC) transporter
389
1591228..1592352
CCC13826_2276
Zonula occludens toxin (Zot) family protein
374
1592354..1592800
CCC13826_2277
Hypothetical protein
148
1592797..1594923
CCC13826_0183
Hypothetical protein
708
1594955..1595095
CCC13826_0184
Hypothetical protein
46
1595477..1595698
CCC13826_2278
Phosphonate uptake transporter
73
1596412..1596648
CCC13826_0164
Sensory box protein
78
1596649..1597101
CCC13826_2078
Hypothetical protein
150
1597203..1598111
CCC13826_2077
Phage integrase
302
1598360..1599571
CCC13826_0020
Putative phage replication protein A
403
1599620..1600789
CCC13826_0019
ABC transporter
389
1600833..1601957
CCC13826_2075
Zot family protein
374
1601959..1602405
CCC13826_2074
Hypothetical protein
148
1602402..1604528
CCC13826_1299
Hypothetical protein
708
1604560..1604700
CCC13826_1298
Hypothetical protein
46
1605082..1605303
CCC13826_2279
Phosphonate uptake transporter
73
1606017..1606253
CCC13826_0185
Sensory box protein
78
1606254..1606706
CCC13826_0186
Hypothetical protein
150
1606808..1607701
CCC13826_0706
Phage integrase
297
1607701..1608312
CCC13826_0188
Glutathionylspermidine synthase family
203
1608327..1609667
CCC13826_0189
Bacteriophage replication gene A protein
446
1609899..1611041
CCC13826_0190
Type II and III secretion system protein
380
1610992..1612116
CCC13826_0191
Hypothetical protein
374
1612118..1612438
CCC13826_0192
Hypothetical protein
106
1612537..1613946
CCC13826_0193
Hypothetical protein
469
1613956..1614075
CCC13826_0194
Alkyl hydroperoxide reductase
39
1614090..1614209
CCC13826_0195
Hypothetical protein
39
1614489..1614707
CCC13826_0196
Hypothetical protein
72
1614801..1615178
CCC13826_0197
Hypothetical protein
125
Interestingly, the region that was considered as an “incomplete prophage” by Stothard et al[33] turned out to be four putative prophages, each beginning with a phage integrase (Figure 3 and Table 4). The first prophage had a genome size of 5.2 kb, which contained seven protein-encoding genes. The second prophage and third prophage were identical, each having a genome size of 9.6 kb consisting of 10 protein-encoding genes. The fourth prophage contained 11 protein-encoding genes with a genome size of 8.6 kb. We named the first prophage CON_phi1, the second prophage and third prophage CON_phi2 and the fourth prophage CON_phi3. The zot gene is a component of CON_phi2. Comparison of the proteins encoded by genes in CON_phi2 with that encoded by genes in CTX phage, the phage that carries the zot gene in V. cholera, did not show high similarities except for the Zot protein (data not shown), suggesting the CON_phi2 is a previously uncharacterised prophage.
A study by Kaakoush et al[36] found that two hypothetical proteins encoded by CCC13826_0191 and CCC13826_1210 have 47% and 46% similarity respectively to C. concisus Zot. Here we found that CCC13826_0191 is a gene of CON_phi3 and CCC13826_1210 is a gene of an additional putative prophage, which was named CON_phi4. A number of genes in CON_phi3 and CON_phi4 had high similarities, however, CON_phi4 did not contain the gene that corresponding to CCC13826_0188 in CON_phi3 (Table 5).
Table 5 The similarity of proteins in CON_phi3 and CON_phi4.
IDENTIFICATION OF CONSERVED MOTIFS SHARED BY C. CONCISUS ZOT AND ZONULIN/ZOT RECEPTOR BINDING DOMAINS
Kaakoush et al[36] compared Zot sequences in C. concisus strain 13826 and V. cholerae strain 86015 and reported that the biological active domain (FCIGRL) previously found in V. cholerae Zot was not found in C. concisus Zot. In this review, we compared the sequence of C. concisus Zot with human zonulin receptor binding domain and V. cholerae Zot receptor binding domain previously reported[32,37]. Interestingly, we found that C. concisus Zot shares conserved motifs with both the human zonulin receptor binding domain and the V. cholerae Zot receptor binding domain (Table 6). These data suggest that C. concisus Zot may increase intestinal permeability using a mechanism that is similar to the human zonulin and V. cholerae Zot, affecting the tight junctions through proteinase activated receptor 2 activation[37,38]. The motif “GRFLSYHG” is located at amino acid position 123-130 in C. concisus Zot, which was found in all oral zot-positive C. concisus strains that we previously isolated as well as in the C. concisus strain 13826[14]. The polymorphisms of C. concisus zot gene that Mahendran et al[14] previously detected were not in the receptor binding domain, suggesting that these polymorphisms may impact on the function of C. concisus Zot, if there is any, using a different mechanism rather than affecting the binding of C. concicsus Zot to the receptor.
Table 6 Motifs shared by Campylobacter concisus zonula occludens toxin and zonulin/zonula occludens toxin receptor binding domains.
INCREASED INTESTINAL PERMEABILITY IN PATIENTS WITH IBD
Increased intestinal permeability is a feature of both CD and UC[39-43]. While epithelial cell death and proinflammatory cytokines may damage the intestinal epithelial barrier during active disease, evidence shows that increased intestinal permeability may precede the initial onset or relapse of IBD. An early study from Hollander et al[39] reported that increased intestinal permeability was detected not only in patients with CD but also in their healthy relatives. A family history of IBD is a known risk factor for IBD[44]. Irvine et al[41] reported that an individual with a family history of CD had elevated intestinal permeability eight years prior to the onset of clinical symptoms and diagnosis of CD. Wyatt et al[43] measured the intestinal permeability in patients with quiescent CD and found that those with increased intestinal permeability were at a significantly higher risk of clinical relapse. These data suggest that increased intestinal permeability occurred prior to the onset and relapse of the disease may be a possible aetiological factor of IBD.
C. CONCISUS ZOT: A POTENTIAL TRIGGER OF IBD THROUGH CAUSING PRIMARY BARRIER DEFECT
The human zonulin and V. cholerae Zot toxin are known to increase intestinal permeability through affecting the tight junctions[31,32,37]. In this review, we found that C. concisus Zot has conserved motifs shared by the zonulin/Zot binding receptor domains. Given this, it is very likely that C. concisus Zot also affects the tight junctions.
Based on the information obtained from previous publications and the analysis that we have performed in this review, we propose a mechanism by which C. concisus, an oral bacterium, may trigger the onset or relapse of IBD: that some oral C. concisus strains acquire zot gene from a virus (prophage). With the human oral cavity as the reservoir of C. concisus, repeated intestinal colonization of C. concisus and release of C. concisus Zot due to prophage induction may occur, which is likely to result in a prolonged primary epithelial barrier defect and translocation of macromolecule such as luminal microbes and their products. In genetically susceptible individuals, this may trigger the development of IBD.
Damage to the intestinal epithelial tight junctions may also lead to the development of diarrhea. Indeed, in addition to its association with IBD, C. concisus has been frequently isolated from non-IBD-related diarrheal stool samples[9,45,46].
If some oral C. concisus strains are indeed involved in the development of human IBD, the question as to why the lesions of IBD occur more often in the intestinal tract rather than in the oral cavity arises. One explanation is that the virulence factors that are associated with IBD are more often expressed in the intestinal tract rather than in the oral cavity. For example, the expression of C. concisus Zot may require induction of prophage from the C. concisus genome. As prophage induction usually occurs when bacterial cells are under stressful conditions[47], the fact that C. concisus uses the human oral cavity as its primary colonization site suggests that the oral cavity is not a stressful site for C. concisus. However, as the C. concisus travels to the more hostile lower parts of the gastrointestinal tract, the stressful environment may trigger the induction of C. concisus prophage.
Another possible factor that may reduce the pathogenic effect of C. concisus Zot in the oral cavity is that the epithelium in the oral cavity is a stratified squamous epithelium, either keratinized or non-keratinized[48]. In contrast, the intestinal epithelium is a simple columnar epithelium[48]. The impact on permeability caused by Zot, even it is expressed in the oral cavity, in multiple layers of squamous epithelium may not be as evident as that in the single layered columnar epithelium.
C. CONCISUS ZOT: A POTENTIAL ENVIRONMENTAL FACTOR CONTRIBUTING TO THE INCREASED RISK OF IBD IN INDIVIDUALS WITH A FAMILY HISTORY OF IBD
A family history of IBD is a risk factor for developing IBD[44]. In addition to genetic factors, environmental factors have been shown to be involved in the increased incidence of IBD in members with a family history of this disease[49,50]. We suggest that C. concisus Zot is one such factor. This suggestion is based on the findings that the higher numbers of the relatives of patients with IBD have increased intestinal permeability and that some oral C. concisus strains carry the zot gene that encodes a toxin known to promote this[14,39,41]. This hypothesis remains to be further assessed by examining the correlation between colonization of zot-positive C. concisus strains and the increased intestinal permeability in family members of patients with IBD.
CONCLUSION
The evidence presented in this review shows that some C. concisus strains colonizing the human oral cavity acquired zot gene from a virus (prophage). We are currently examining the biologic activities of C. concisus Zot, the expression of Zot in zot-positive C. concisus strains isolated from patients with IBD and controls as well as the presence of C. concisus Zot in the oral cavity and intestinal tract of patients with IBD and controls, which will provide further information in understanding the role of C. concisus Zot in IBD and other human diseases.
ACKNOWLEDGMENTS
The authors would like to thank Vikneswari Mahendran and Jenny Norman for providing the scanning electron microscopic picture of C. concisus.
Footnotes
P- Reviewers: Actis GC, Azuma YT, Capasso R S- Editor: Gou SX L- Editor: A E- Editor: Wang CH
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