Effect of GP73 silencing on proliferation and apoptosis in hepatocellular cancer

Figure 2 Expression of GP73 mRNA and protein for Hep G2 and Bel7402 cells after transfection with Stealth RNAi against GP73, negative control RNAi or blank control. A: Relative expression of GP73 mRNA level at 24 h after transfection analyzed by quantitative real-time reverse transcription polymerase chain reaction (RT-PCR); B: Relative expression of GP73 mRNA level at 48 h after transfection analyzed by quantitative RT-PCR; C: Expression of GP73 proteins in supernatant of Hep G2 and Bel7402 cells were detected by enzyme-linked immunosorbent assay (ELISA) at 24 and 48 h; D: Expression of GP73 proteins in supernatant of Hep G2 and Bel7402 cells was detected by ELISA at 48 h. The corresponding values of GP73 protein in the three groups at 24 and 48 h after transfection. ^bP < 0.01 vs blank group.