Brief Reports
Copyright ©The Author(s) 2003.
World J Gastroenterol. Sep 15, 2003; 9(9): 2114-2116
Published online Sep 15, 2003. doi: 10.3748/wjg.v9.i9.2114
Figure 1
Figure 1 Comparative binding reaction of β2GPI to rHBsAg by ELISA-based determination of protein interaction.
Figure 2
Figure 2 Comparative binding reaction of β2GPI to BSA by ELISA-based determination of protein interaction.
Figure 3
Figure 3 Reduced (lane1) and non-reduced (lane2) human β2GPI (1 μg/lane) was separated with 12% SDS-PAGE and applied to Hybond nitrocellous membrane, blocked with 5% skimmed milk, then probed with biotinylated rHBsAg, color was developed with HRP-avidin in DAB solution. Single band at -50 kDa was observed, no significant diference in color den-sity was observd, which was further confirmed by digital scaning.