Brief Reports
Copyright ©The Author(s) 2003.
World J Gastroenterol. Aug 15, 2003; 9(8): 1844-1847
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1844
Figure 1
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Figure 1 Structure of alb-Cre-ERt expression vector. The DNA fragments contained albumin gene promoter/enhancer (alb e/p), rabbit β-globin intron, fusion gene of Cre-ERt, and polyadenylation site (pA).
Figure 2
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Figure 2 Identification of palb-Cre-ERt recombinant. M: GeneRuler 1 kb DNA Laddar, 1: recombinant-HindIII, 2: recombinant-ScaI, 3: recombinant-EcoRV, 4:Vector-HindIII.
Figure 3
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Figure 3 A part of mice was screened by Southern blot analysis. P: positive plasmid, 1,2,4: positive mice(2kb), 3: negative mice.
Figure 4
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Figure 4 Electrophoresis analysis of PCR products amplified from DNA obtained from F1 mice tail tissues. Lanes 1-4 (transgenic mice) : positive bands (600 bp), lane 5: normal mouse (negative control), lane 6: blank control, M: Generuler of 1Kb DNA landar.
Figure 5
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Figure 5 Electrophoresis analysis of RT-PCR products from transgenic mice. Lane 1: kidney tissue, lane 2: brain tissue, lane 3: liver (505 bp), lane 4: lung, M: Generuler of 100 bp DNA laddar.