Relationship between telomerase activity and its subunit expression and inhibitory effect of antisense hTR on pancreatic carcinoma

Figure 1 Telomerase activity of fibroblasts and P₃ cells of dif-ferent generations (1, 3 and 5 were P₃ cells of the 10^th, 20^th, and 30^th generations; 2, 4, and 6 were fibroblasts of the 10^th, 20^th, and 30^th generations).

Figure 2 Specificity and sensitivity of telomerase activities in P3 cells (1, 293 cells as the positive control; 2,104 P₃ cells; 3, treated with RNase; 4, heat treated; 5, 6, 7, and 8 were 103, 102, 101, and 100 P₃ cells, respectively; 9, the lytic liquid).

Figure 3 a: Telomerase activity of tumor and normal pancreas (T: tumor; N: normal). b: Semiquantitative telomerase activity of tumor and normal pancreas (1 2 3; 5 4 6; 9 10 11: T₁₀, T₁₅ T₁₈ 6 μg 0.6 μg 0.06 μg; 7: negative control, 8: positive control; T: tumor).

Figure 4 Telomerase subunit expression of pancreatic carci-noma (1: P3; 2: PaTu-8801; 3: fibroblast cell line; 4, 5, 7: tumor; 6, 8: normal).

Figure 5 Telomerase activity after treated with PS-ODN in P3 cell line (1: positive control; 2: negative control; 3-6: 100 μmol/ L, 31. 60 μmol/L, 10 μmol/L, 3.16 μmol/L PS-ODN1 groups; 7-10: 3.16 μmol/L,10 μmol/L, 31.60 μmol/L, 100 μmol/L PS-ODN2 group; 11: 100 μmol/L PS-ODN3 group; 12: 100 μmol/L PS-ODN4 groups).

Figure 6 The apoptotic rate of P3 cells by the FCM test after treatment in P₃ cells with PS-ODN. A: PS-ODN1 10 μmol/L group; B: PS-ODN1 100 μmol/L group.