cagA and vacA genotype of Helicobacter pylori associated with gastric diseases in Xi’an area

Figure 1 Restriction endonuclease EcoRI, HindIII digests of PcagA. 1. λDNA HindIIImarker, 2. PcagA, 3. Fragement di-gested by EcoRI-HindIII, 4. PUC18/MSPI marker.

Figure 2 Colony hybridization detection of cagA. The positive hybridization dot.

Figure 3 PCR typing of vacA signal sequence. 1.PCR marker, 2. Type s2, 3. Type s1, 4. Standard strain 86313, 5. Standard strain 60 190.

Figure 4 PCR typing of vacA s1a. 1. PCR marker, 2. Clinical isolates, 3. Standard strain 84 183.

Figure 5 PCR typing of vacA mid-region. 1. PCR marker, 2. Clinical isolates, 3. Standard strain 84 183.

Figure 6 PCR typing of vacA s1a. 1. PCR marker, 2.Type m2, 3. Type m1, 4. Standard strain 86 313, 5. Standard strain 60 190.