Basic Research
Copyright ©The Author(s) 2003.
World J Gastroenterol. Jul 15, 2003; 9(7): 1571-1575
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1571
Figure 1
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Figure 1 Structure of maxizyme against mtp53.
Figure 2
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Figure 2 Steps of pU6Mz construction.
Figure 3
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Figure 3 Restrictive enzyme analysis of recombinant plasmid pU6Mz by Sma I and Sac I. M was DNA Marker (DL2000). 1, 2, 3 were selected clones.
Figure 4
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Figure 4 In vitro transcripts. (A) In vitro transcripts of wtp53 and mtp53 (1002nt). (B) In vitro transcripts of maxizyme and control maxizyme (910nt). lane 1: mtp53; lane 2: wtp53; lane 3: maxizyme; lane 4: control maxizyme.
Figure 5
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Figure 5 In vitro cleavage reaction. lane 1: cleavage of wtp53 by cold maxizyme; lane 2: cleavage of mtp53 by inactive cold maxizyme; lane 3: cleavage of mtp53 by cold maxizyme.