Maxizyme-mediated specific inhibition on mutant-type p53 in vitro

Figure 1 Structure of maxizyme against mtp53.

Figure 2 Steps of pU6Mz construction.

Figure 3 Restrictive enzyme analysis of recombinant plasmid pU6Mz by Sma I and Sac I. M was DNA Marker (DL2000). 1, 2, 3 were selected clones.

Figure 4 In vitro transcripts. (A) In vitro transcripts of wtp53 and mtp53 (1002nt). (B) In vitro transcripts of maxizyme and control maxizyme (910nt). lane 1: mtp53; lane 2: wtp53; lane 3: maxizyme; lane 4: control maxizyme.

Figure 5 In vitro cleavage reaction. lane 1: cleavage of wtp53 by cold maxizyme; lane 2: cleavage of mtp53 by inactive cold maxizyme; lane 3: cleavage of mtp53 by cold maxizyme.