Expression of metallothionein gene at different time in testicular interstitial cells and liver of rats treated with cadmium

Figure 1 DNA sequences of RT-PCR products from rat testis.

Figure 2 Time course of Cd accumulation in liver after a single subcutaneous injection of 4 μmol Cd/kg. The values were the mean S.E. (n = 3). An asterisk indicated a significant difference (*P < 0.05 vs control) from the untreated.

Figure 3 Effect of cadmium treatment on MT1(a) and MT2(b) mRNA levels in testicular interstitial cells (B) and the liver (A) of untreated rats (0h) and Cd-treated rats after 1 h, 3 h, 6 h, or 24 h (lanes 1-5). Values were obtained by RT-PCR analysis followed by densitometry scanning and expressed as MT1(C) or MT2(D) mRNA RT-PCR products to β-actin mRNA RT-PCR products ratio. Data represented the mean SE (n = 9, three treatment replicates with three analyses per replicate).

Figure 4 Metallothionein protein in testicular interstitial cells and liver of untreated and Cd-treated rats. Experimental conditions are shown in Figure 3. The values were the mean S.E. (n = 3).