Characteristics and application of established luciferase hepatoma cell line that responds to dioxin-like chemicals

Figure 1 Fragment mapping analysis of recombinant plasmid digested with BglII and HindIII in gel electrophoresis. Lane 1: pGL₃ plasmid was cleaved into two fragments, which showed about 5000 bp and 200 bp respectively. Lane 2: Recombinant plasmid was cut into a fragment of about 5000 bp and the one of about 1000 bp. Lane 3: DNA marker.

Figure 2 The time course of luciferase activities induced by TCDD and DMSO in stably transfected cells. The TCDD-induced luciferase activity in HepG₂-Luc cells peaked at about 4 h, and then decreased to a stable level at about 14 h after TCDD treatment.

Figure 3 Responsive period of HepG₂-Luc cells to TCDD induction. The responsiveness of HepG₂-Luc cells to TCDD induction was decreased with culture time and became undetectable at about 10^th month.

Figure 4 Comparison of luciferase activity induced by TCDD and 3, 3', 4, 4'-PCB in HepG₂-Luc cells. The luciferase activity induced by indicated concentrations of 3, 3', 4, 4'-PCB was much less than that induced by TCDD.