cDNA suppression subtraction library for screening down-regulated genes in gastric carcinoma

Figure 1 Size range of mRNA on 2% agarose gel electrophorsis. Normal gastric mucosa mRNA (lane1) and gastric carcinoma mRNA (lane 2). 100 bp size marker (lane M).

Figure 2 Digested and undigested double strands cDNA products. Undigested double strands cDNA products (lane 1) and RsaI digested double strands cDNA products (lane 2). 100bp size marker (lane M).

Figure 3 Detection of adaptors ligation efficiency. Tester1-1 was amplified with G3PDH3' primer, PCR primer1 (lane 1) and tester1-1 with G3PDH3’, 5'primers (lane 2). Tester1-2 was amplified with G3PDH3'primer, PCR primer1 (lane 3) and tester1-2 with G3PDH3’, 5'primers (lane 4). 100 bp size marker (lane M).

Figure 4 Analyses of PCR products. Secondary PCR products of subtraction samples (lane 1) and unsubtraction samples (lane 2). 100 bp size marker (lane M).

Figure 5 Identification of subtraction efficiency by PCR. G3PDH PCR products of subtracted samples at 18, 23, 28 and 33 cycles (lanes1, 2, 3 and 4) respectively and G3PDH PCR products of unsubtracted samples respectively at 18, 23, 28 and 33 cycles (lanes 5, 6, 7 and 8). 100 bp size marker (lane M).

Figure 6 Identification of inserted fragments in plasmids of library by PCR. Inserted fragments in plasmids of library (lanes 1-10). 100 bp size marker (lane M).