Carbonic anhydrase isozymes IX and XII in gastric tumors

Figure 1 The principle for calculation of the relative staining index in one normal gastric sample (LS = luminal surface, PZ = proliferative zone, G = gastric glands).

Figure 2 Immunohistochemical staining of CA IX in normal gastric mucosa (A), metaplasia (B), adenoma with moderate dysplasia (C), adenocarcinoma grade II (D), adenocarcinoma grade III (E), and diffuse carcinoma (F). In the normal mucosa, CA IX was expressed in all histological layers covering the luminal surface (LS), proliferative zone (PZ), and gastric glands (G). It was notable that CA IX was confined to deep crypts in metaplastic epithelium. The adenoma sample and all malignant lesions shown in this figure were positive for CA IX. (Original magnifications × 200).

Figure 3 The mean staining indices for CA IX in the normal gastric mucosa and different gastric lesions (P = 0. 002, normal vs dysplasia, adenocarcinoma, and metaplasia).

Figure 4 The mean staining indices for CA IX in the normal gastric mucosa and lesions grouped according to the stages of dysplasia and grades of malignancy (P < 0. 05, normal vs gd, gr. II and gr. III).

Figure 5 Immunohistochemical staining of CA XII in normal gastric mucosa (A), metaplasia (B), adenoma with moderate dysplasia (C), adenocarcinoma grade II (D), adenocarcinoma grade III (E), and diffuse carcinoma (F). From these samples, CA XII was not expressed in the normal epithelium and showed weak expression in metaplastic epithelium and diffuse carcinoma. The signal was stronger in adenoma and adenocarcinoma samples.

Figure 6 The mean staining indices for CA XII in the normal gastric mucosa and different gastric lesions (P < 0. 05, normal vs adenocarcinoma).

Figure 7 The mean staining indices for CA XII in the normal gastric mucosa and lesions grouped according to the stages of dysplasia and grades of malignancy (P = 0. 003, normal vs gr. II).