Expression and cell-specific localization of cholecystokinin receptors in rat lung

Figure 1 Appearance of positive and negative control tissue section for detecting in situ expression of CCK-AR and CCK-BR gene by in situ RT-PCR. (A) In situ expression of CCK-BR gene in the brain of SD rats (× 400); (B) In situ expression of CCK-AR gene in the brain of SD rats (× 400); (C) Negative control section from the lung tissue of SD rats (× 200).

Figure 2 Localization of CCK-BR gene in the lung tissues of SD rats by in situ RT-PCR (× 400). (A) Expression of CCK-BR gene in bronchial mucosal cells; (B) Expression of CCK-BR gene in vascular endothelial cells; (C) Expression of CCK-BR gene in macrophages and alveolar epithelial cells.

Figure 3 Localization of CCK-BR mRNA in rat lung tissues by in situ hybridization. (A) Expression of CCK-BR gene in vascular endothelial cells (× 100); (B) Expression of CCK-BR gene in vascular endothelial cells and macrophages (× 400).

Figure 4 In situ expression of CCK-AR gene in the lung tissues of SD rats, detected by in situ RT-PCR (× 400). (A) Expression of CCK-AR gene in vascular endothelial cells; (B) Expression of CCK-AR gene in bronchial mucosal cells and macrophages; (C)Expression of CCK-AR gene in alveolar epithelial cells.

Figure 5 No positive signal of CCK-AR gene was detected in lung tissues by in situ hybridization (× 200).