Mix-infections with different genotypes of HCV and with HCV plus other hepatitis viruses in patients with hepatitis C in China

Figure 1 Alignment of DNA sequence of the 5’NCR of HCV isolated from the patients mix-infected with genotypes 1 and 3 mix-infected patients. Comparison of nucleotide sequences in the 5’NCR from Chinese patients (YS-, R-) with previously published HCV sequences. Nucleotide numbering corresponds to that described for the prototype HCV1[9]. Dashes indicate identity with sequence of HCV1 (top line). Nucleotide substitutions are indicated. Numbers in parentheses indicates the genotype of that sequence. Source of published sequences referred to Materials and Methods.

Figure 2 Alignment of DNA sequence of the 5'NCR of HCV isolated from the patients mix-infected with different variants of the same genotype. Comparison of nucleotide sequences in the 5’NCR from Chinese patients (YS-, R-) with previously published HCV sequences. Patients were presumably divided in three groups, as shown in the left column. Nucleotide numbering corre-sponds to that described for the prototype HCV1[9]. Dashes indicate identity with sequence of HCV1 (top line). Nucleotide substitutions are indicated. Numbers in parentheses indicates the genotype of that sequence. Source of published sequences referred to Materials and Methods.

Figure 3 Phylogentic tree constructed with Chinese (YS-, R-) and previously reported sequences, based on nucleotide (nt) identity of 5’NCR sequence (nt -220 to -47). Dentrogram shows 19 Chinese isolates distributed within 2 major genotypes (genotypes 1 and 3). Source of published sequences referred to Materials and Methods.

Figure 4 Phylogentic tree constructed with Chinese (YS-, R-) and previously reported sequences, based on nucleotide (nt) identity of 5’NCR sequence (nt -220 to -47). Dentrogram shows 34 different Chinese isolates distributed within 3 major genotypes, 14 isolates in genotype 1, 4 in genotype 2, and 16 in genotype 3. Source of published sequences referred to materials and Methods.