Synergistic effects of nimesulide and 5-fluorouracil on tumor growth and apoptosis in the implanted hepatoma in mice

Figure 1 Electro micrographs of nimesulide plus 5-FU treated mice hepatoma. A, control; B, nimesulide plus 5-FU.

Figure 2 DNA ladder pattern of hepatoma tissues as demon-strated by agarose gel 1. 5% electrophoresis. M, DNA markers; lanes 0-3, control, nimesulide 20 mg/kg, 5-FU 20 mg/kg, nimesulide 20 mg/kg + 5-FU 10 mg/kg.

Figure 3 Data of flow cytometry of mouse hepatoma without any treatment as a control (A), or following 21 d treatment with nimesulide 20 mg/kg (B), 5-FU 20 mg/kg (C) and nimesulide 20 mg/kg + 5-FU 10 mg/kg (D). The sub-G1 peak to the left of the G1 peak represents apoptotic cells.

Figure 4 Effect of Nimesulide and 5-FU on PGE₂ content in mouse hepatoma. Each column is the mean ± SD of the sample. 1-4, control, nimesulide 20 mg/kg, 5-FU 20 mg/kg, and nimesulide 20 mg/kg + 5-FU 10 mg/kg. n = 3. ^-x±s. ^aP < 0.01 vs control, ^cP < 0.01 vs nimesulide + 5-FU.

Figure 5 Effect of nimesulide and 5-FU on expression of c-fos, c-jun, and p53 in mouse hepatoma. 0-3, control, nimesulide 20 mg/kg, 5-FU 20 mg/kg, nimesulide 10 mg/kg + 5-Fu 10 mg/kg. The β-actin was used as an intrinsic reference molecule.