Differentially expressed proteins of gamma-ray irradiated mouse intestinal epithelial cells by two-dimensional electrophoresis and MALDI-TOF mass spectrometry

Figure 1 Histological changes in crypts following irradiation. A: Unirradiated controls (×100). B: 3 hours after a single dose of 9.0 Gy (×100). Villi and crypts remained unchanged, but cell division of intestinal epithelia ceased. C: 3 days after a single dose of 9.0 Gy (×100).

Figure 2 Two-dimensional electrophoresis maps. A: normal intestinal epithelial cells, B: 3 h irradiated epithelial cells, C: 72 h post-radiation. Number indicated proteins were summarized in Table 2.

Figure 3 MALDI-TOF-MS spectrum of spot P35700 in 2-DE map. MS spectrum of peptide mixture was obtained from a typical in-gel digestion of the 2-DE separated protein.

Figure 4 Western blot analysis of Enolase after radiation. A: Protein samples, obtained at indicated time after radiation, were separated by SDS-PAGE, and immunoblotted with corresponding antibody. Equal amounts of total proteins were applied to each lane. B: Relative protein level of Enolase was determined by quantitating the intensity of Enolase bands with a densitometer.

Figure 5 Semi-quantitative RT-PCR analysis of peroxiredoxin I. A: PCR product run on an agarose gel. The upper band repre-sented peroxiredoxin I (750 bp), and the lower band represented GAPDH (450 bp). B: Relative abundance of the mRNA of peroxiredoxin I was normalized by GAPDH.

Figure 6 Semi-quantitative RT-PCR analysis of Q8VC72. RNA samples were prepared from mouse intestinal epithelia at indi-cated time. Left: The band represents Q8VC72 (461 bp). Right: The band represents GAPDH (450 bp). The expression of Q8VC72 remained unchanged after radiation.