Gene and protein expressions of p28GANK in rat with liver regeneration

Figure 1 Northern blot of p28^GANK mRNA in rat regenerating liver tissues showed two transcripts of 1. 5 kb and 1.0 kb. Expression level of p28^GANK mRNA increased 2 h after PH, and reached the peak level at 72 h. More significant variation was found for 1.5 kb transcript.

Figure 2 No significant difference of the expression of p28^GANK mRNA was seen in the SO group. In the PH group, the expres-sion increased in 1.5 kb transcript 2 h after PH. The peak time was at 72 h, but gradually decreased after 72 h.

Figure 3 The expression of p28^GANK protein in regenerating liver tissues was examined by immunohistochemistry. A: Local brown expression of p28^GANK in the cytoplasm and nucleus of regenerating hepatocyte near central region 24 h after PH. IHC × 400; B: Diffuse brown expression of p28^GANK in the cytoplasm and nucleus of regenerating hepatocyte 48 h after PH. IHC × 400; C: Regional light yellow expression of p28^GANK in cytoplasm of regenerating hepatocyte 72 h after PH. IHC × 400; D: Dispersed light yellow expression of p28^GANK in cytoplasm of regenerating hepatocyte 120 h after PH. IHC × 400.