Studies on mechanism of Sialy Lewis-X antigen in liver metastases of human colorectal carcinoma

Figure 1 The expression of α1,3Fuc-TmRNA in Lovo cells shows strongly positive staining, with deep royal blue cytoplasms and visible royal blue beads. In situ hybridization. × 400

Figure 2 The expression of α1,3Fuc-TmRNA shows weakly positive staining, light royal blue with royal blue bead in cytoplasm of HT29 cells. In situ hybridization. × 400

Figure 3 The expression of SLeX antigen in Lovo cells shows strongly positive staining, with deep brown in cytiplasm. Immunohistochemistry. × 400

Figure 4 The expression of SLeX antigen shows positive or weakly positive staining, brown in membrane of HT29 cells. Immunohistoc hemistry. × 400

Figure 5 The detective result of Lovo cells. flow cytometry

Figure 6 The detective result of HT29 cells. flow cytometry

Figure 7 In control groups, a lot of Lovo cells adhered to the surface of umbilical vein endothelium, and tumor cells clustered. SEM, × 500

Figure 8 In control groups, The HT29 cells adhered to the surface of umbilical vein endothelium, a part of them clustered. SEM, × 500

Figure 9 In experimental groups, a small amount of the Lovo cells adhered to the umbilical vein endothelium, no clustering. SEM, × 500

Figure 10 In experimental groups, a small amount of the HT29 cells adhered to the umbilical vein endothelium, no clustering. SEM, × 500

Figure 11 The Lovo cells were linked to umbilical vein endothelium via interdigitations on the cell membrane. TEM × 20K

Figure 12 The HT29 cells connected endothelial cell membrane directly via the cell membrane. TEM × 24K