Brief Reports
Copyright ©The Author(s) 2000.
World J Gastroenterol. Oct 15, 2000; 6(5): 734-737
Published online Oct 15, 2000. doi: 10.3748/wjg.v6.i5.734
Figure 1
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Figure 1 a: The HBV genomic sequence, cloned in pBR322. Positions of restriction site (B, BamH I; Bs, BstE II; X, Xba I; Xh, Xho I) b: 0.65 kb fragment, containing the 3'-truncated preS/S. c: Construction of the vector (pcDNA3.1-preS/S) for expressing 3'-truncated preS/S.
Figure 2
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Figure 2 The pcDNA3. 1 digested with Nhe I, Pme I, Xba I and Xho I. 1: Nhe + Xba I; 2: Xho I + Nhe I; 3: Pme I + Xba I; M: λ/EcoR I+ Hind-III marker
Figure 3
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Figure 3 The DNA analysis of founder mice by PCR. 324 bp fragment was amplified with positive and negative primers. 1: blank control; 2: PCR for genomic DNA of normal mice; 3: PCR for genomic DNA of transgenic mice (No.1); 4: PCR for genomic DNA of transgenic mice (No.3); M: λ/Eco RI + Hind III marker