Transduction of primary rat hepatocytes with bicistronic retroviral vector

Figure 1 Structure of bicistronic retroviral vector PGCEN/β-gal. Arrow below vector indicates initiated site of transcription.

Figure 2 Morphology of hepatocytes in culture. × 100 Hepatocytes became polygonal epitheliumlike structure. The majority of cells were mononucleated; Some cells were bi-or multi-nucleated. The membranes were visible. Hepatocytes became confluent at 4 d postplating.

Figure 3 Transduction efficiency of hepatocytes by retroviral vector.

Figure 4 PCR detection of NeoR in primary rat hepatocytes transduced by PGCEN/β-gal. A: Size markers (Lambda DNA/EcoR + Hind III Marker) B: Positive template (MN45Li cell lines modified by NeoR gene) C: Primary rat hepatocytes transduced with retroviral vector PGCEN/β-gal D: Nontransduced primary rat hepatocytes E: Water control