Suppression of P-gp induced multiple drug resistance in a drug resistant gastric cancer cell line by overexpression of Fas

Figure 1 SDS-PAGE and Western blot of Fas in three kinds of cell lines. A: SDS-PAGE B: Western blot 1. Total protein (100 μg) extracted from pBK-SGC7901/VCR 2. Total protein (150 μg) extracted from pBK-SGC7901/VCR 3. Marker (M_r× 10³) 4. Total protein (100 mg) extracted from fas-SGC7901/VCR 5. Total protein (150 mg) extracted from fas-SGC7901/VCR

Figure 2 Survival rates of three kinds of cells treated with chemotherapeutic drugs (%). ^aP < 0.05, vs fas-SGC7901/VCR

Figure 3 Agarose gel electrophoresis of apoptoctic cells DNA. 1, 2: pBK-SGC7901/VCR; 3, 4: fas-SGC7901/VCR; 1, 3: VM-26 (1.25 μmol/L); 2, 4: VM-26 (2.5 μmol/L)

Figure 4 Detection of P-gp expression in the 3 tumor cells lines by immunocytochemical staining. × 400 A: pBK-SGC7901/VCR; B: SGC7901; C: fas-SGC7901/VCR