Stereoselective propranolol metabolism in two drug induced rat hepatic microsomes

Figure 1 Chromatograms of propranolol after incubati on with rat hepatic microsome. A Shim-pack CLC-ODS column (15 cm × 0.6 cm i.d.) was used. The mobile phase was constituted with ammonium acetate buffer (pH 4.0)-methanol (50:50) with flow rate at 1.0 mL/min. Propranolol was monitored at 290nm. Propranolol: ^tR = 10.1 min.

Figure 2 Concentration time curves for R (+) and S (-)-propranolol metabolism in rat hepatic microsomes. A. Microsome of control. B. Microsome induced by BNF. C. Microsome induced by PB. △—△: R( + )-propranolol. ○—○: S (-)-propranolol.