Preparation and purification of F(ab’)2 fragment from anti hepatoma mouse IgG1 mAb

Figure 1 Photograph of SDS-PAGE get of anti-hepato ma IgG₁ mAb HAb18 before and after digestion and purification. Lane 1, molecul ar weight standards; lane 2-3, reduced and non-reduced mAb HAb18; lane 4, papai n digest of HAb18; lane 5-6, purified F(ab’)₂ fragment under non-reduction and reduction conditions.

Figure 2 Purification of F(ab’)₂ fragme nt from papain digest of mAb HAb18 by FPLC using DEAE-Sepharose-FF column.