Protective effect of Irsogladine on monochloramine induced gastric mucosal lesions in rats: a comparative study with rebamipide

Figure 1 Effects of irsogladine and rebamipide on ga stric lesions induced by NH₂Cl in rats. The animals were given NH₂Cl (120 mM:5 mL/kg) po, and sacrificed 1 h later. Irsogladine (1 mg/kg-10 mg/kg ) or rebamipide ( 30, 100 mg/kg) was given po 30 min before administration of NH₂Cl. Data are presented as the mean ± SE fr om 5-7 rats. ^aStatistically significant difference from control (P < 0.05).

Figure 2 Effects of indomethacin and L-NAME on the mucosal protective action of irsogladine and rebamipide against NH₂Cl-induced gastric lesions in rats. The animals were administered po with 1 mL of NH₂Cl (120 mM), and sacrificed 1 h later. Irsogladine (3 mg/kg) or rebamipide (100 mg/kg) was given po 30 min before NH₂Cl treat ment. Indomethacin (5 mg/kg, sc) or L-NAME (10 mg/kg, iv) was given 30 or 10 min before the above agents. Data are presented as the mean ± SE from 5-8 rats. Statistically significant difference at P < 0.05; *from the corresponding control (CMC);#from the corresponding value in normal group.

Figure 3 Effects of irsogladine and rebamipide on ch anges in transmucosal PD in response to NH₂Cl in anesthetized rat stomachs. Th e stomach was mounted on an ex-vivo chamber, and NH₂Cl (20 mM; 1 mL) was applied topically to the stomach for 10min. Irsogladine (3 mg/kg) or rebamipide (100 mg/kg) was applied to the chamber for 30 min, starting 20 min before exposure to NH₂Cl. Data are presented as the mean ± SE of value sdetermined every 5 min from 5-7 rats. ^aStatistically significant differen ce from control, P < 0.05.

Figure 4 Effects of irsogladine and rebamipide on changes in transmucosal PD in response to NH₄OH in rat stomachs under ischemic conditions. The stomach mounted on a ex-vivo chamber was subjected to ische mia by bleeding from the carotid artery (1 mL/100 g body wt), and then e xposed to NH₄OH (120 mM; 1 mL) for 1h thereafter. Irsogladine (3 mg/kg) or rebamipide (100 mg/kg) was applied to the chamber 20 min before the onset of ischemia and NH₄OH treatment. Indomethacin (5 mg/kg, sc) or L-NAME (10 mg/kg, iv) was given 30 or 10 min before the above agents. Data are presented as the mean ± SE of values determined every 5 min from 5-6 rats. Statistically significant difference at P < 0.05; *from control; #from vehicle.

Figure 5 Effects of irsogladine and rebamipide on ga stric mucosal lesions induced by NH₄OH in anesthetized rat stomachs under ischemic conditions. The stomach mounted on an ex-vivo chamber was subjected to ischemia by bleeding from the carotid artery (1 mL/100 g body weight), and then exposed to NH₄OH (120 mM) for 1 h thereafter. Irsogladine (3 mg/kg) or rebamipide (100 mg/kg)- was applied to the chamber 20 min before the onset of ischemia and NH₄OH treatment. Indomethacin (5 mg/kg, sc ) or L-NAME ( 10 mg/kg, iv ) was given 30 or 10 min before the above agents. Data are presented as the mean ± SE from 4-6 rats. Sta tistically significant difference at P < 0.05; *from control;^#f rom vehicle.