HBeAg gene expression with baculovirus vector in silk worm cells

Figure 1 Partial sequence of HBeAg amplified by PCR.

Figure 2 Construction of recombinant vector pBm HBe.

Figure 3 Restriction endonuclease analysis plasmid pBm HBe (Left). 1. p Bm HBe/Bgl II, linerization; 2. λDNA/Hind III marker; 3. p Bm Hbe/Bg-II + Sma 1 I, released 0.54 kb HBeAg gene.

Figure 4 PAG Electrophoresis analysis of HBeAg prote in expressed in Bm N cells(Right). 1 and 2: Bm N cells infected withr Bm-HBe, the arrow shows HBeAg protein, M_r18000; 3: Standard protein molecular weight marker, M_r94000, 17000, 43000, 20000, 17500; 4 and 5: Bm- N cells infected with wt Bm NPV, the arrow shows M_r32000 polyhe dea protein; 6: Culture supernatant, the arrow shows HBeAg protein.

Figure 5 Structure of HBeAg gene and PCR design.