Transduction of Fas gene or Bcl-2 antisense RNA sensitizes cultured drug resistant gastric cancer cells to chemotherapeutic drugs

Figure 1 Identification of recombinant vectors digested with enzymes. a. pBluescript Fas/Xho I, b. pBluescript Fas/Eco R I+Sal I, c. pBK-Fas/Eco RI+Sal I, d. γ DNA Marker/Hind III, e. pDOR-Bcl-2/Eco R I+Bam H I, f. pBluescript Bcl-2/Eco R I+Bam H I, g. pBluescript Bcl-2/Eco R I

Figure 2 Electrophoresis of pBK-Fas-PCR product. a. PCR of competent cells as template, b. PCR of empty vector transformed bacterial, c. PCR marker, d. PCR1 of pBK-Fas (500bp), e. PCR2 of pBK-Fas (850 bp), f. PCR3 of pBK-Fas (700 bp)

Figure 3 Northern blot with Fas- cDNA probe. a. SGC7901/VCR/3 × 10⁶ cells, b. empty vector transfectant/5 × 10⁶ cells, c. SGC7901/3 × 10⁶ cells, d. RNA Marker, e. SGC7901 -Fas/VCR/1 × 10⁶ cells, f. SGC7901 -Fas/VCR/2 × 10⁶ cells, g. SGC7901 Fas/VCR/3 × 10⁶ cells.

Figure 4 Northern blot with Bcl-2 probe. A. antisense probe, B. sense probea. a. SGC7901/3× 10⁶ cells, b. empty vector transfectant/2 × 10⁶ cells c. SGC7901/3 × 106 cells, d. RNA Marker, e. SGC7901 anti-Bcl-2/VCR/2 ×10⁶ cells, f. SGC7901 anti-Bcl-2/VCR/3 × 10⁶ cells, g. SGC7901 anti Bcl-2/VCR/4 × 10⁶ cells

Figure 5 SDS-PAGE and Western blot. A. Fas transfectants, B. anti -Bcl-2 transfectants. a. SGC7901/VCR/3 × 10⁶ cells, b. SGC7901/3 × 10⁶ cells, c. High molecular weight protein marker, d. SGC7901-Fas/VCR/1 × 10⁶ cells, e. SGC7901-Fas/VCR/2 × 10⁶ cells, f. SGC7901/1 × 10⁶ cells, g. SGC7901/2 × 10⁶ cells, h. SGC7901/VCR/1 × 10⁶ cells, i. SGC7901/VCR/2 × 10⁶ cells, j. Middle molecular weight protein marker, k. SGC7901 anti Bcl-2/VCR/5 × 10⁵ cells, l. SGC7901 anti-Bcl-2/VCR/1 × 10⁶ cells, m. SGC7901 anti-Bcl-2/ VCR/2 × 10⁶ cells, n. SGC7901 anti Bcl-2/VCR/3 × 10⁶ cells