Gene therapy for human colorectal carcinoma using human CEA promoter controled bacterial ADP-ribosylating toxin genes: PEA and DTA gene transfer

Figure 1 Structure of the retroviral constructs. LTR: long terminal repeat region; Neo: neomycin resistance gene; CMV: human cytomegalovirus immediate early promoter; CEA: human carcinoembryonic antigen promoter; PEA: Pseudomonas exotoxin domain II + III fragment; DTA: diphtheria toxin A.

Figure 2 Inhibition of luciferase gene expression by the toxin gene cotransfection. A: Transfection with pGL2-Control only; B: Cotransfection with pGL2-Control and G1CEAPEANa; C: Cotransfection with pGL2-control and G1CEADTANa; D: Cotransfection with pGL2-control and pLNCPEA; E: Cotransfection with pGL2-control and pLNCDTA.