Copyright
©The Author(s) 1998.
World J Gastroenterol. Oct 15, 1998; 4(5): 385-387
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.i5.385
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.i5.385
Figure 1 Effect of growth phase on the competence of natural transformation in H.
pylori. H38 DNA (50 mg/L) was added to NCTC 11637 at the indicated time intervals. Total cell number and transformants were enumerated.
Figure 2 Dependence of transformation frequency on the concentration of donor H38 DNA.
H38 DNA with different concentration was added to 108 NCTC 11637. Number of transformants was enumerated.
Figure 3 Comparison of DNA profiles of transformants and parent strains by RAPD.
PCR products were run on 1% agarose gel. Strains H13, H41 and H43 were metronidazole sensitive. Lanes 1 & 8. λ DNA digested with Hind III. Lanes 2 & 3. H13 and its transformant. Lanes 4 & 5. H41 & its transformant. Lanes 6 & 7. H43 and its transformant.
- Citation: Hua JS, Zheng PY, Teo KF, Khin MM, Ho B. Helicobacter pylori acquistion of metronidazole resistance by natural transformation in vitro. World J Gastroenterol 1998; 4(5): 385-387
- URL: https://www.wjgnet.com/1007-9327/full/v4/i5/385.htm
- DOI: https://dx.doi.org/10.3748/wjg.v4.i5.385