Helicobacter pylori acquistion of metronidazole resistance by natural transformation in vitro

doi:10.3748/wjg.v4.i5.385

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Oct 15, 1998 (publication date) through Nov 20, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 15, 1998; 4(5): 385-387
Published online Oct 15, 1998. doi: 10.3748/wjg.v4.i5.385

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Figure 1 Effect of growth phase on the competence of natural transformation in H. pylori. H38 DNA (50 mg/L) was added to NCTC 11637 at the indicated time intervals. Total cell number and transformants were enumerated.

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Figure 2 Dependence of transformation frequency on the concentration of donor H38 DNA. H38 DNA with different concentration was added to 108 NCTC 11637. Number of transformants was enumerated.

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Figure 3 Comparison of DNA profiles of transformants and parent strains by RAPD. PCR products were run on 1% agarose gel. Strains H13, H41 and H43 were metronidazole sensitive. Lanes 1 & 8. λ DNA digested with Hind III. Lanes 2 & 3. H13 and its transformant. Lanes 4 & 5. H41 & its transformant. Lanes 6 & 7. H43 and its transformant.

Citation: Hua JS, Zheng PY, Teo KF, Khin MM, Ho B. Helicobacter pylori acquistion of metronidazole resistance by natural transformation in vitro. World J Gastroenterol 1998; 4(5): 385-387
URL: https://www.wjgnet.com/1007-9327/full/v4/i5/385.htm
DOI: https://dx.doi.org/10.3748/wjg.v4.i5.385