Simplified isolation and spheroidal aggregate culture of rat hepatocytes

Figure 1 Phase-contrast microscopic feature of multicellular spheroidal aggregates of hepatocytes at day 4. ( × 200)

Figure 2 Scanning electron microscopy of spheroidal aggregates at day 14. ( × 1000)

Figure 3 Transmisson electron microscopy of hepatocyte spheroids cultured for 25 days, showing abundant organelles in hepatocytes. ( × 10000)

Figure 4 Phase-contrast photomicrograph of hepatocyte spheroids attached to collagen-coated wells. ( × 100)

Figure 5 Albumin synthesis and secretion by aggregate hepatocytes.