Mesalazine alleviated the symptoms of spontaneous colitis in interleukin-10 knockout mice by regulating the STAT3/NF-κB signaling pathway

Figure 1 Effect of mesalazine on alleviating symptoms of spontaneous colitis in interleukin-10^-/- mice. A: Daily mouse body weight; B: Disease activity index score; C: Colon length and statistical chart; D: Histological image of the colon of the mouse (hematoxylin & eosin, magnification × 100). The wild group and interleukin-10^-/- group were given normal saline every day and the mice in the 5-amino salicylic acid group were given mesalazine every day. n = 6-8. Compared with the interleukin-10^-/- group, ^aP < 0.05. WT: Wild type; IL: Interleukin; ASA: Amino salicylic acid; NS: No significance.

Figure 2 Effect of mesalazine on alleviating endoplasmic reticulum stress in interleukin-10^-/- mouse colonic epithelial cells. A: Transmission electron microscope photograph of mouse colonic epithelial cells (magnification × 8200); B: Glucose-regulated protein 78 protein level; C: Representative images of glucose-regulated protein 78 immunofluorescence (green) and nuclei (blue) in colonic tissue; D: Representative images of C/EBP homologous protein immunofluorescence (green) and nuclei (blue) in colonic tissue. Compared with the interleukin-10^-/- group, ^aP < 0.05, ^bP < 0.01. WT: Wild type; IL: Interleukin; ASA: Amino salicylic acid; GRP78: Glucose-regulated protein 78; DAPI: 4’,6-diamidino-2-phenylindole; CHOP: C/EBP homologous protein.

Figure 3 Regulation of protein and gene expression related to signal transducer and activator of transcription 3/nuclear factor kappa B signaling pathway in colonic tissue by mesalazine. A: P-signal transducer and activator of transcription 3 protein level; B: Nuclear factor kappa B protein level; C: p-IκB protein level; D: Signal transducer and activator of transcription 3 and nuclear factor kappa B gene levels. Compared with the IL-10^-/- group, ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. STAT3: Signal transducer and activator of transcription 3; WT: Wild type; IL: Interleukin; ASA: Amino salicylic acid; NF-κB: Nuclear factor kappa B.