Hepatitis G virus infection in patients with chronic non-A–E hepatitis

Figure 1 Electrophoretic pattern of RT-nested PCR for hepatitis G virus (HGV) RNA detection. 1, 4 were products of PCR from original sera, 2 and 5 products from 10^-6 diluted sera, 3 and 6 were negative controls. 1, 2 and 3 were the frist PCR products, 4, 5 and 6 were the second PCR products. M was DNA marker, PBR 322/BstN I.

Figure 2 RT-nested PCR for amplification of long fragment cDNA of hepatitis G virus (HGV) NS5 region. 1 was negative control, 2 was positive PCR product, M was DNA and PCR marker.

Figure 3 Clinical manifestation of the patient with chronic non-A-E hepatitis

Figure 4 Nucleotide sequence of partial hepatitis G virus (HGV) NS5 gene in the serum of a patient with chronic non-A-E hepatitis and its comparison with West African and American isolates. GBV-C is West African isolate; R10291 and PNF2161 are American isolates; HG-G is a patient with chronic non-A-E hepatitis. —denotes the same as GBV-C sequence. Capital letter is nucleotide detected, small letter is primer sequence.

Figure 5 Amino acid sequence of hepatitis G virus (HGV) NS5 region in the serum of the patient with chronic non-A-E hepatitis and its comparison with West African and American isolates.