Ultrastructural observation of liver tissue ablation induced by high-intensity focused ultrasound

Figure 1 Beam characteristics of the 1. 1 MHz high intensity focused ultrasound transducer.

Figure 2 Light micrography showing the cytoplasmic glycogen decreased in ablated lesion (c, p) immediately after high intensity focused ultrasound (HIFU) exposure by stained PAS. N = Normal liver; C = Lesion centre; P = Lesion edge. × 66.

Figure 3 Electron micrography of a normal rabbit hepatocyte. N = Nucleus; M = Mitochondria; RER = Rough endoplasmic reticulum; G = Glycogen.

Figure 4 Electron micrography from the edge region of the lesion immediately after HIFU treatment showing the destruction of organelles and massive vacuolization of the cytoplasm as a sign of irreversible cell death.

Figure 5 Electron micrography from the centre of the lesion, showing complete cellular disruption with karyorrhexis and many electronic deposits existed and cavities left.