Adiponectin and the regulation of gastric content volume in the newborn rat

Figure 1 Gastric emptying rate (panel A) and related postprandial content volume (panel B) for one- (n = 30), two- (n = 10) and three-weeks old (n = 16) fasting rats[5]. Data are mean ± SE; ^aP < 0.01 vs other postprandial times; ^bP < 0.01 vs other ages by two-way ANOVA with multiple comparisons evaluated by the Tukey-Kramer test. Citation: Ferreira CHF, Shifrin Y, Pan J, Ivanovska J, McNamara PJ, Belik J. The newborn rat gastric emptying rate is volume and not developmentally dependent. Neurogastroenterology and motility: 2018; 30: e13233. Copyright© The Authors 2018. Published by Neurogastroenterol Motil. The authors have obtained the permission for figure using from the Publishing Group (Supplementary material).

Figure 2 Smooth muscle cells shortening in response to carbachol stimulation (carbachol) in the absence and presence of adiponectin (ADPN + carbachol). Rat fundic smooth muscle cells (SMC) were freshly obtained from one- (n = 178: carbachol; n = 198: ADPN + carbachol), two-weeks (n = 109: carbachol; n = 93: ADPN + carbachol) old pups and adult rats (n = 924: carbachol; n = 564: ADPN + carbachol). Data are expressed as mean ± SE percentage of basal length measurements prior to contraction. ^aP < 0.01 vs respective ADPN + carbachol. Insert shows representative SMC length measurements (magnification: 20 ×) under the different conditions. SMC: Smooth muscle cells.

Figure 3 Newborn rat smooth muscle cell length expressed as a percentage of basal (non-stimulated; n = 178) length following pre-incubation with adiponectin and carbachol stimulation in the absence (n = 198) and pre-incubation presence of Iberiotoxin, a large-conductance Ca²⁺ sensitive K⁺ channel inhibitor (n = 96). Data are mean ± SE ^aP < 0.01 vs basal smooth muscle cell length values. SMC: Smooth muscle cells.

Figure 4 Freshly obtained, one-week old (n = 6) and two-weeks old (n = 8) rat milk curd adiponectin content. Data are shown as individual values and mean ± SE ^aP < 0.01 vs two-weeks old values.