Genetic association analysis of CLEC5A and CLEC7A gene single-nucleotide polymorphisms and Crohn’s disease

Figure 1 Effects of the rs1285933 genotype on CLEC5A and CXCL5 gene expression. Peripheral blood mononuclear cells were isolated from individuals with genotype AA (n = 8), GG (n = 5), and AG (n = 9), cultured and treated with lipopolysaccharide (1 µg/mL) for 6 h. Subsequently, the mRNA expression of the indicated genes and the house-keeping control GAPDH was analyzed by real-time PCR. Data are presented as averaged ΔCt values ± standard error of mean. ^aP < 0.05 vs genotype GG.