Basic Study
Copyright ©The Author(s) 2019.
World J Gastroenterol. Jul 28, 2019; 25(28): 3775-3786
Published online Jul 28, 2019. doi: 10.3748/wjg.v25.i28.3775
Figure 1
Figure 1 The miRNA expression profile in pancreatic cancer. A: The volcano plot was constructed using P-values and fold change values; B: The clustered heatmap showed the top 20 differentially expressed miRNAs between the control and pancreatic cancer groups. Red indicates the up-regulated expression and green/blue indicates the down-regulated expression.
Figure 2
Figure 2 Detection of expression of miR-205 in pancreatic cancer tissues and cell lines by qRT-PCR. A: MiR-205 was up-regulated in pancreatic cancer tissues (n = 41); B: MiR-205 was up-regulated in pancreatic cancer cell lines (PANC-1, BXPC-3, and SW1990) compared with normal human pancreatic duct epithelial cell line HPDE6 (P < 0.05); C: MTT assay. Cell viability was significantly increased in cells with overexpression of miR-205 compared with control cells in a time-dependent manner; D: Colony formation assay. The effect of miR-205 overexpression on cell proliferation was evaluated.
Figure 3
Figure 3 GO pathway analysis of the target genes of miR-205.
Figure 4
Figure 4 Pathway analysis of the target genes of miR-205.
Figure 5
Figure 5 Expression of adenomatous polyposis coli in pancreatic cancer cell lines. A: mRNA level; B: Protein level, P < 0.05. APC: Adenomatous polyposis coli.
Figure 6
Figure 6 MiR-205 promotes proliferation of pancreatic cancer cells by targeting adenomatous polyposis coli. A: Predicted binding sites of miR-205 in adenomatous polyposis coli (APC). The fragment of wild-type or mutant 3’-UTR of APC was cloned into the luciferase reporter vector; B: Luciferase activities measured at 48 h post transfection; C: APC mRNA expression was decreased in miR-205 mimic treated PANC-1 cells but increased in miR-205 inhibitor treated cells; D and E: The promoting effect of miR-205 on proliferation was reduced in pancreatic cancer cells overexpressing APC, as measured by MTT and colony formation assays. APC: Adenomatous polyposis coli; wt: Wild-type; mut: Mutant.