Autoimmune liver disease-related autoantibodies in patients with biliary atresia

Figure 1 Representative strips after color development by line-blot immunoassay. The line-blot immunoassay strips had been coated with nine autoimmune liver disease-related antigens, including Ro-52, SLA/LP, LC-1, LKM-1, gp210, PML, Sp100, BPO and AMA-M2 (from left to right). BA group: ALD/93-57 with anti-BPO +; ALD/87-31 with anti-Ro-52 +++, anti-PML +, anti-Sp100 +, anti-BPO ++, and AMA-M2 +; ALD/89-53 with anti-BPO +; Other liver diseases group: Only ALD/198-59 with anti-LC-1 ±; Healthy group: All autoantibodies were negative. Positive control (ALD/52-75) showed anti-BPO +++ and AMA-M2 +++. Other liver diseases include choledochal cysts, transient cholestasis of unknown origin, and neonatal intrahepatic cholestasis caused by citrin deficiency. ALD: Autoimmune liver disease; BA: Biliary atresia; LC-1: Liver cytosolic antigen type 1.

Figure 2 Positivity of anti-M2-3E detected by enzyme linked immunosorbent assay in biliary atresia patients and controls. ^aP < 0.05 vs healthy controls; ^cP < 0.05 vs other liver diseases. Other liver diseases include choledochal cysts, transient cholestasis of unknown origin, and neonatal intrahepatic cholestasis caused by citrin deficiency. BA: Biliary atresia; ELISA: Enzyme linked immunosorbent assay.

Figure 3 The main fluorescence patterns of anti-neutrophil cytoplasmic antibodies in biliary atresia patients. ANCA detection by indirect immunofluorescence assays was performed on ethanol-fixed (upper panel) and formaldehyde-fixed (lower panel) human neutrophils. Depending on whether reactivities of formaldehyde-fixed human neutrophils were positive or not, p-ANCA was divided into p-ANCA with formaldehyde resistance and p-ANCA with formaldehyde sensitivity. ANCA: Anti-neutrophil cytoplasmic antibody; BA: Biliary atresia; (c)-ANCA: Cytoplasmic-ANCA; (p)-ANCA: Perinuclear-ANCA.