Gut microbial diversity analysis using Illumina sequencing for functional dyspepsia with liver depression-spleen deficiency syndrome and the interventional Xiaoyaosan in a rat model

Figure 1 Saccharine preference index of the normal group (FD1) and the model group (FD2). The data are presented as mean ± SE. ^aP < 0.01 vs the normal group.

Figure 2 Agarose gel electrophoresis of PCR products of the V4 region of 16S rDNA. Normal group: FD (1.1-1.3); Model group: FD (2.1-2.3); Xiaoyaosan group: FD (3.1-3.3). FD: Functional dyspepsia.

Figure 3 Statistical graph of Tags and Operational Taxonomic Unit clustering. Normal group: FD (1.1-1.3); Model group: FD (2.1-2.3); Xiaoyaosan group: FD (3.1-3.3). Total Tags: Effective Tags; Unique Tags: Uneffective Tags; Taxon Tags: Annotated Tags for OTUs; Unclassified Tags: Unannotated Tags. OTUs: Operational Taxonomic Units; FD: Functional dyspepsia.

Figure 4 Relative abundance of the microbiomes at the phylum level. Normal group: FD1; Model group: FD2; Xiaoyaosan group: FD3. FD: Functional dyspepsia.

Figure 5 Heat map of species abundance clustering at the phylum level. Normal group: FD1; Model group: FD2; Xiaoyaosan group: FD3. FD: Functional dyspepsia.