Therapeutic effect of curcumin on experimental colitis mediated by inhibiting CD8+CD11c+ cells

Figure 1 Macroscopic and microscopic observation. A: Macrography of the opened colon; B: Body weight; C: DAI score; D: Colonic weight; E: Weight index of the colon; F: Typical histological images stained by hematoxylin and eosin, f1-4: Bar = 40 μm, f5-8: Bar = 100 μm; G: Colonic length; H: Histological scores. Data are presented as mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs Normal group; ^cP < 0.05 and ^dP < 0.01 vs TNBS group.

Figure 2 Typical histograms and levels of CD8⁺CD11c⁺ cells in the spleen and PPs. A: Typical graphs and mean fluorescence intensity (MFI) levels of CD8⁺CD11c⁺ cells in the spleen; B: Typical graphs and MFI levels of CD8⁺CD11c⁺ cells in PPs; C: MFI levels of CD8⁺CD11c⁺ cells in the spleen; D: MFI levels of CD8⁺CD11c⁺ cells in PPs. Data are shown as mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs Normal group; ^cP < 0.05 and ^dP < 0.01 vs TNBS group.

Figure 3 Levels of TGF-β1, IFN-γ and IL-10 in spleen and colonic mucosal supernatants. A-C: Concentration of TGF-β1, IFN-γ and IL-10 in the spleen from different groups; D-F: Concentration of TGF-β1, IFN-γ and IL-10 in the colonic mucosa from different groups. Data are shown as mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs Normal group; ^cP < 0.05 and ^dP < 0.01 vs TNBS group.

Figure 4 Typical histograms and levels of CD11c⁺CD40⁺ cells in spleen and PPs. A: Typical graphs of CD11c⁺CD40⁺ cells in the spleen; B: Typical graphs of CD11c⁺CD40⁺ cells in the PPs; C: MFI levels of CD11c⁺CD40⁺ cells in the spleen; D: MFI levels of CD11c⁺CD40⁺ cells in the PPs. Data are described as mean ± SEM (n = 8). ^aP < 0.05 vs Normal control group; ^cP < 0.05 vs TNBS group.

Figure 5 Typical histograms and levels of CD11c⁺CD40L⁺ cell in spleen and PPs. A: Typical graphs of CD11c⁺CD40L⁺ cells in the spleen; B: Typical graphs of CD11c⁺ CD40L⁺ cells in PPs; C: Levels (MFI) of CD11c⁺CD40L⁺ cells in the spleen; D: Levels (MFI) of CD11c⁺CD40L⁺ cells in PPs. Data are described as mean ± SEM (n = 8). ^aP < 0.05 vs Normal control group; ^cP < 0.05 vs TNBS group.

Figure 6 Typical histograms and levels of CD11c⁺ ICAM-1⁺ cells in spleen and PPs. A: Typical graphs of CD11c⁺ICAM-1⁺ cells in the spleen; B: Typical graphs of CD11c⁺ICAM-1⁺ cells in PPs; C: Levels (MFI) of CD11c⁺ ICAM-1⁺ cells in the spleen; D: Levels (MFI) of CD11c⁺ICAM-1⁺ cells in the PPs. Data are shown as mean ± SEM (n = 8). ^aP < 0.05 vs Normal control group; ^cP < 0.05 and ^dP < 0.01 vs TNBS group.

Figure 7 Typical histograms and levels of CD11c⁺CD205⁺ cells in the spleen and PPs. A: Typical graphs of CD11c⁺ CD205 ⁺ cells in the spleen; B: Typical graphs of CD11c⁺CD205⁺ cells in the PPs; C: Levels (MFI) of CD11c⁺CD205⁺ cells in the spleen; D: Levels (MFI) of CD11c⁺CD205⁺ cells in PPs. Data are mean ± SEM (n = 8). ^aP < 0.05 and ^bP < 0.01 vs Normal group; ^cP < 0.05 vs TNBS group.

Figure 8 Typical histograms and levels of CD11c⁺MHC II⁺ cells in the spleen and PPs. A: Typical graphs of CD11c⁺ MHC II⁺ cells in the spleen; B: Typical graphs of CD11c⁺ MHC II⁺ cells in the PPs; C: Levels (MFI) of CD11c⁺ MHC II⁺ cells in the spleen; D: Levels (MFI) of CD11c⁺ MHC II⁺ cells in the PPs. Data are mean ± SEM (n = 8). ^aP < 0.05 vs Normal control group; ^cP < 0.05 and ^dP < 0.01 vs TNBS group.