Semaphorin 4D and hypoxia-inducible factor-1α overexpression is related to prognosis in colorectal carcinoma

Figure 1 Hypoxia-inducible factor-1α expression patterns. A: A sample with strong cytoplasmic staining for hypoxia-inducible factor-1α (HIF-1α) in the advancing tumor border is shown; interstitial cells are positive for HIF-1α (× 200); B: Cancer tissue with positive immunostaining for HIF-1α (× 400); C: Cancer tissue with negative immunostaining for HIF-1α (× 200); D: HIF-1α is not expressed in normal tissue (× 200).

Figure 2 Expression patterns of semaphorin 4D. A: A sample with strong cytoplasmic staining for semaphorin 4D (Sema4D) in the advancing tumor border is shown, with interstitial cells being positive for Sema4D (× 200); B: Cancer tissue with positive immunostaining for Sema4D (× 400); C: Cancer tissue with negative immunostaining for Sema4D (× 200); D: Sema4D is not expressed in normal tissue (× 200).

Figure 3 Cumulative survival curves. Cumulative survival curves were stratified by semaphorin 4D (Sema4D) and hypoxia-inducible factor-1α (HIF-1α) expression in cancer cells. The survival curves were analyzed by Kaplan-Meier analysis and log-rank tests. A: Colorectal carcinoma patients with positive Sema4D expression exhibited shorter overall survival compared with patients with negative expression (P = 0.009); B: Colorectal carcinoma patients with positive HIF-1α expression exhibited shorter overall survival compared with those with negative expression (P = 0.497). Neither value reached statistical significance.