Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Jul 21, 2015; 21(27): 8314-8325
Published online Jul 21, 2015. doi: 10.3748/wjg.v21.i27.8314
Figure 1
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Figure 1 High-mobility group protein 1-toll-like receptor 4 and downstream signaling pathways. IFN: Interferon; IL: Interluekin; MyD88: Myeloid differentiation gene 88; NF-κB: Nuclear factor-κB; TNF: Tumor necrosis factor; TRIF: Translocating-chain-associating membrane protein; HMGB1: High-mobility group protein 1; TLR4: Toll-like receptor 4.
Figure 2
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Figure 2 Levels of myeloperoxidase activity in the lung and liver. S: Sham group; C: Control group; H: Anti-high-mobility group protein 1 group; M: Anti-myeloid differentiation gene 88 group; T: Anti-translocating-chain-associating membrane protein group; n = 4; aP < 0.05 vs sham group; bP < 0.05 vs control group; cP < 0.05 vs anti-HMGB1 group. MPO: Myeloperoxidase.
Figure 3
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Figure 3 Hematoxylin and eosin staining of lungs in all groups (magnification × 400). A: Sham group; B: Control group; C: Anti-high-mobility group protein 1 group; D: Anti-myeloid differentiation gene 88 group; E: Anti-translocating-chain-associating membrane protein group (magnification × 400; scale bars = 50 μm).
Figure 4
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Figure 4 Hematoxylin and eosin staining of the jejunum and ileum in all groups (magnification × 100). Representative images from jejunum (A1-E1) and ileum (A2-E2). A: Sham group; B: Control group; C: Anti-high-mobility group protein 1 group; D: Anti-myeloid differentiation gene 88 group; E: Anti-translocating-chain-associating membrane protein group (magnification × 100; scale bars = 200 μm).
Figure 5
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Figure 5 Expression of HMGB1 and NF-κB in lung, jejunum, and ileum in all groups. HMGB1 expression in: Lung (A1); Jejunum (A2); and Ileum (A3); respectively, in all groups; NF-κB expression in: Lung (B1); Jejunum (B2); and Ileum (B3). S: Sham group; C: Control group; H: Anti-HMGB1 group; M: Anti-myeloid differentiation gene 88 group; T: Anti-translocating-chain-associating membrane protein group. n = 4,aP < 0.05 vs sham group; bP < 0.05 vs control group; cP < 0.05 vs anti-HMGB1 group. HMGB1: High-mobility group protein 1; NF-κB: Nuclear factor-κB.