Primary analysis and screening of microRNAs in gastric cancer side population cells

Figure 1 Side population cell analysis. The P3 gate was the side population (SP) cells and the P4 gate was the major population (MP) cells. A: SP ratio in MKN-45 was 2.0%; B: The SP cells obviously decreased with both Hoechst 33342 and verapamil. Figure 1 cited partly from the article of Not All Side Population Cells Contain Cancer Stem-Like Cells in Human Gastric Cancer Cell Lines which published in Dig Dis Sci 2013; 58: 132-139.

Figure 2 MicroRNA microarray. Affymetrix genechip 2.0 was used to examine microRNA expression profiles of SP and MP cells of MKN-45. A: Hybridized genechip with microRNA of SP cells; B: Hybridized genechip with microRNA of MP cells. SP: Side population; MP: Major population.

Figure 3 Visualization of the global microRNA regulated network. Red nodes represent microRNA, yellow nodes represent coding genes. Green edge represents the microRNA-target relationship, blue edge represents the protein-protein interaction.