Beneficial effect of an omega-6 PUFA-rich diet in non-steroidal anti-inflammatory drug-induced mucosal damage in the murine small intestine

Figure 1 Histological evaluation of impact of indomethacin and fat-rich diets. Representative microscopic images of the small intestine are shown. Control diet (R) without indomethacin (IND) (A), with IND (B), SFA-rich diet (BT) without IND (C), with IND (D), omega-3 PUFA-rich diet (FO) without IND (E), with IND (F), and omega-6 PUFA-rich diet without IND (G), with IND (H).

Figure 2 Effect of dietary fat treatment on the recruitment of monocytes to submucosal venules. A: Change over time of monocyte adhesion in the submucosal venules of the small intestine; B: Representative photographs of in vivo monocyte adherence to small intestinal microvessels of IND and control diet (R)-treated animals; C: Representative photographs of in vivo monocyte adherence to small intestinal microvessels of IND and omega-6 PUFA diet (SO)-treated animals (n = 8 in each group). Values are represented as mean ± SEM, n = 8; ^aP < 0.05 vs IND + control diet (R); ^cP < 0.05 vs IND + omega-6 PUFA diet (SO).

Figure 3 Effect of dietary fat treatment on the recruitment of monocytes to postcapillary venules. A: Change over time of monocyte adhesion in the postcapillary venules of the small intestine; B: Representative photographs of in vivo monocyte adherence to small intestinal microvessels of IND + control diet (R)-treated animals; C: Representative photographs of in vivo monocyte adherence to small intestinal microvessels of IND + omega-6 PUFA diet (SO)-treated animals (n = 8 in each group). Values are represented as mean ± SEM, n = 8; ^aP < 0.05 vs control diet (R), ^cP < 0.05 vs IND + control diet (R), ^eP < 0.05 IND + omega-6 PUFA diet (SO).

Figure 4 Effect of dietary fat treatment on recruitment of platelets to submucosal venules. Time-course change in platelet adhesion in the submucosal venules of the small intestine. ^aP < 0.05 vs control diet (R), ^cP < 0.05 vs IND + control diet (R), ^eP < 0.05 vs IND + omega-6 PUFA diet (SO).