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World J Gastroenterol. Aug 21, 2014; 20(31): 10886-10894
Published online Aug 21, 2014. doi: 10.3748/wjg.v20.i31.10886
Published online Aug 21, 2014. doi: 10.3748/wjg.v20.i31.10886
Figure 1 Colonic transit increased in intragastric cold water stressed rats.
Control group: the rats treated with intragastric room temperature physiological saline; Cold group: the rats treated with intragastric cold physiological saline. aP < 0.05 vs cold and control rats (n = 10).
Figure 2 Effects of oxytocin (0.
01-0.3 μmol/L) on colon contraction in intragastric cold water stressed rats. A: The dose-dependent contractile response to oxytocin (OT) in colonic smooth muscle strips in cold and control groups. OT was administered at the points marked by the arrows. B: Average response to OT in the colon. aP < 0.05 vs the data prior to OT administration. cP < 0.05 vs cold and control groups (n = 6).
Figure 3 Atosiban (0.
1 mmol/L) blocked oxytocin-induced colonic response in intragastric cold water stressed rats. A: Representative traces of the effect of atosiban on oxytocin (OT)-induced colonic response in the cold group. The drug was administered at the points marked by the arrows. B: Results of colonic smooth muscle strips contraction in cold group rats. aP < 0.05 vs the data of atosiban treatment (n = 8).
Figure 4 Tetrodotoxin (10 μmol/L) blocked oxytocin-induced colonic response in intragastric cold water stressed rats.
A: Representative traces of the effect of tetrodotoxin (TTX) on oxytocin (OT)-induced colonic response in cold group. The drug was administered at the points marked by the arrows. B: Results of colonic smooth muscle strips contraction in cold group rats. aP < 0.05 vs data of TTX treatment (n = 6).
Figure 5 Intragastric cold water stress increased oxytocin receptor expression in colon.
A: Representative Western blots. B: Densitometry analysis. C: Positive control of immunoreactivity. Oxytocin (OT) receptor antigen is expressed in the smooth muscle (arrows) of uterus. D: OT receptor immunoreactivity is expressed in myenteric plexus (arrows) of colon. aP < 0.05 vs control group (n = 6). OTR: Oxytocin receptors.
Figure 6 Estradiol regulated the plasma concentration of oxytocin in intragastric cold water stressed rats.
A: Weight of uterus. B: The plasma concentration of oxytocin (OT) in the four groups [control, cold, ovariectomy (OVX) + cold and OVX + E2 + cold]. Data are expressed as mean ± SD (n = 6). aP < 0.05 vs control group. cP < 0.05 vs cold group. eP < 0.05 vs OVX + cold group.
Figure 7 Ovariectomy decreased the oxytocin-induced inhibition of colonic contractility in intragastric cold water stressed rats.
A: Representative traces of the effect of ovariectomy (OVX) on oxytocin (OT)-induced colonic response in cold group rats. The drug was administered at the points marked by the arrows. OT-induced inhibition of colonic strips decreased after the rats were ovariectomized. B: The contraction of colonic smooth muscle strips in cold group rats. aP < 0.05 vs the data in normal Krebs solution. cP < 0.05 vs cold group and OVX + cold group (n = 6).
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Citation: Yang X, Xi TF, Li YX, Wang HH, Qin Y, Zhang JP, Cai WT, Huang MT, Shen JQ, Fan XM, Shi XZ, Xie DP. Oxytocin decreases colonic motility of cold water stressed rats
via oxytocin receptors. World J Gastroenterol 2014; 20(31): 10886-10894 - URL: https://www.wjgnet.com/1007-9327/full/v20/i31/10886.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i31.10886