Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection

Figure 1 Representative example of the dual-priming oligonucleotide-based multiplex polymerase chain reaction. Lane M: Amplicon size marker (Seegene Inc.,Korea); Lane 1: Negative; Lane 2: Mutant type of A2143G; Lane 3-4: Wild type; PC1: A2142G mutant positive control; PC2 : A2143G mutant positive control; NC: Negative control.

Figure 2 Detection of Helicobacter pylori and the A2143G/A2142G of the 23S rRNA gene on the basis of the dual-priming oligonucleotide-based multiplex polymerase chain reaction product in enrolled patients. M: 100 bp Marker; A: ClaR Marker; B: A2142G Positive Marker; C: A2143G Positive Marker; NC: Negative Marker; 1-54 : Samples.