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World J Gastroenterol. Jun 7, 2014; 20(21): 6547-6553
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6547
Published online Jun 7, 2014. doi: 10.3748/wjg.v20.i21.6547
Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection
Woo Chul Chung, Sung Hoon Jung, Jung Hwan Oh, Tae Ho Kim, Dae Young Cheung, Byung Wook Kim, Sung Soo Kim, Jin Il Kim, Department of Internal Medicine, the Catholic University of Korea, Seoul 130-709, South Korea
Eun Young Sin, The Research Institute of St. Vincent Hospital, the Catholic University of Korea, Suwon 442-723, South Korea
Author contributions: Chung WC and Jung SH designed the study, drafted and revised the manuscript, and approved the final version of the manuscript; Oh JH, Kim TH, Cheung DY and Sin EY collected and analyzed data; Kim BW, Kim SS and Kim JI supervised the study, and extensively revised the manuscript.
Supported by Research grant from Jeil Pharma.Co., Seoul, South Korea
Correspondence to: Sung Hoon Jung, MD, Department of Internal Medicine, the Catholic University of Korea, St. Paul’s Hospital, 180 Wangsan-ro, Dongdaemun-gu, Seoul 130-709, South Korea. shjung74@catholic.ac.kr
Telephone: +82-2-9582114 Fax: +82-2-9687250
Received: July 18, 2013
Revised: December 13, 2013
Accepted: January 8, 2014
Published online: June 7, 2014
Revised: December 13, 2013
Accepted: January 8, 2014
Published online: June 7, 2014
Core Tip
Core tip: The rapid urease test (CLOtest) alone is unreliable in diagnosing Helicobacter pylori (H. pylori) infection and does not provide information about resistance to clarithromycin. Therefore, we investigated whether tissue samples that have been analyzed by the CLOtest kit are suitable for dual-priming oligonucleotide-based multiplex PCR (DPO-PCR) to detect H. pylori. Our results demonstrated that the DPO-based multiplex PCR test using tissue samples processed by the CLO kit is appropriate for detecting H. pylori and clarithromycin resistance. Particularly, in patients with CLO-negative results, this method is helpful for diagnosing H. pylori infection. Moreover, it would be beneficial in economical aspects.