Brief Article
Copyright ©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 28, 2014; 20(16): 4761-4770
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4761
Figure 1
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Figure 1 PCR amplification of the 23S rRNA gene of Helicobacter pylori strains. 1-10: Randomly selected clarithromycin-sensitive strains; 11-20: Randomly selected clarithromycin-resistant strains; M: 100 bp DNA ladder.
Figure 2
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Figure 2 Two new restriction sites for Bbs I and Bsa I caused by clarithromycin resistance-associated mutations A2143G and A2144G in the 23S rRNA gene of Helicobacter pylori strains.
Figure 3
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Figure 3 Digestion of the amplified fragment of the 23S rRNA gene from Helicobacter pylori isolates with restriction endonuclease Bbs I (A) and Bsa I (B). 1-10: Randomly selected clarithromycin-sensitive strains; 11-20: Randomly selected clarithromycin-resistant strains; M: 100 bp DNA ladder.
Figure 4
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Figure 4 Comparison of the 23S rRNA gene sequence of Helicobacter pylori isolates with that of HPJ99 strain. S: A clarithromycin-sensitive isolate; R1-5: clarithromycin-resistant strains.
Figure 5
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Figure 5 REP-PCR analysis of 26 clarithromycin-resistant (A) and clarithromycin-sensitive (B) Helicobacter pylori isolates. M: λDNA/EcoR I-Hind III marker; 1-26: Clarithromycin-resistant Helicobacter pylori isolates; ATCC43504: A standard Helicobacter pylori strain.
Figure 6
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Figure 6 Dendrogram of REP-PCR DNA fingerprints for clarithromycin-resistant Helicobacter pylori strains. A: 6 genotypes; B: 4 genotypes.