Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line

Figure 1 SV40 T-antigen-immortalized mouse hepatic cells (× 200). A: SV40 T-antigen (SV40T)-immortalized mouse hepatic cells visualized by an inverted phase contrast microscope; B: SV40T antigen immunofluorescence in mouse hepatic cells; C: SV40T-immortalized mouse hepatic cells visualized by an electron microscope.

Figure 2 Levels of alanine aminotransferase, aspartate aminotransferase and alpha-fetoprotein in the cell culture supernatant. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; AFP: α-fetoprotein.

Figure 3 Electrophoresis and Western blotting. A: Electrophoresis of determine albumin (ALB) reverse transcription polymerase chain reaction products (1: markers; 2: primary mouse hepatic cells; 3: immortalized mouse hepatic cells at 22^nd generation); B: ALB by Western blotting (1: Markers; 2: Primary mouse hepatic cells; 3: Transfected mouse hepatic cells at 22^nd generation).

Figure 4 Levels of hepatitis B surface antigen and hepatitis B e antigen in the cell culture supernatant. HBsAg: Hepatitis B surface antigen; HBeAg: Hepatitis B e antigen; pHBV1.3: Hepatitis B virus 1.3-fold genome plasmids.

Figure 5 Analysis of hepatitis B surface antigen and hepatitis B c antigen expression in hepatitis B virus 1. 3-fold genome plasmids-transfected cells by immunofluorescence microscopy (× 200). A: Hepatitis B surface antigen (HBsAg) observed in the hepatitis B virus 1.3-fold genome plasmids (pHBV1.3)- transfected cells at 24 h post-transfection; B: Hepatitis B c antigen (HBcAg) observed in the pHBV1.3-transfected cells at 24 h post-transfection; C: HBsAg observed in HepG2.215 cells (positive control); D: HBsAg observed in untransfected SV40 T-antigen (SV40T)-immortalized cells (negative control); E: HBcAg observed in HepG2.215 cells (positive control); F: HBcAg observed in untransfected SV40T-immortalized cells (negative control).

Figure 6 Expression of hepatitis B virus 1. 3-fold genome plasmids in SV40 T-antigen-immortalized mouse hepatic cells. A: 42 nm Dane particles (a) and 22 nm subviral particles (b) in the supernatant; B: Hepatitis B virus (HBV) DNA replication intermediates in HBV 1.3-fold genome plasmids (pHBV1.3)-transfected cells 24 h after transfection. rc DNA: Relaxed circular DNA; dsDNA: Double-stranded DNA; ssDNA: Single-stranded DNA.