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World J Gastroenterol. Nov 28, 2013; 19(44): 8020-8027
Published online Nov 28, 2013. doi: 10.3748/wjg.v19.i44.8020
Published online Nov 28, 2013. doi: 10.3748/wjg.v19.i44.8020
Expression of hepatitis B virus 1.3-fold genome plasmid in an SV40 T-antigen-immortalized mouse hepatic cell line
Xiu-Guang Song, Li-Xian Ma, Department of Infectious Diseases, Qilu Hospital, Shandong University, Jinan 250012, Shandong Province, China
Xiu-Guang Song, Peng-Fei Bian, Shu-Li Yu, Xiu-Hua Zhao, Wei Xu, Xue-Hui Bu, Jinan Infectious Disease Hospital, Shandong University, Jinan 250021, Shandong Province, China
Xia Li, Laboratory for Tumor Immunity and Traditional Chinese Drug Immunity, Institute of Basic Medicine, Shandong Academy of Medical Science, Jinan 250062, Shandong Province, China
Li-Xian Ma, School of Medicine, Shandong University, Jinan 250012, Shandong Province, China
Author contributions: Song XG performed the experiments and drafted the manuscript; Bian PF, Yu SL, Zhao XH, Xu W, Bu XH and Li X helped carry out the experiments; Ma LX helped design the study and revise the manuscript; all authors have read and approved the final manuscript.
Supported by Jinan Science and Technology Bureau, Shandong Province, China, No. 200705095-4
Correspondence to: Li-Xian Ma, MD, Department of Infectious Diseases, Qilu Hospital, Shandong University, No. 107, Wenhua Xilu, Jinan 250012, Shandong Province, China. shandongqilu123@gmail.com
Telephone: +86-531-87933911 Fax: +86-531-87936971
Received: February 16, 2013
Revised: June 27, 2013
Accepted: September 16, 2013
Published online: November 28, 2013
Processing time: 298 Days and 8.1 Hours
Revised: June 27, 2013
Accepted: September 16, 2013
Published online: November 28, 2013
Processing time: 298 Days and 8.1 Hours
Core Tip
Core tip: This study established a new immortalized mouse hepatic cell line through the transfection of the pRSV-T plasmid. SV40 T-antigen (SV40LT)-immortalized mouse hepatic cells had the same morphology and biological characteristics as primary mouse hepatic cells. SV40LT-immortalized mouse hepatic cells could be transfected with the pHBV1.3 plasmid, which caused the hepatitis B virus (HBV) genes to replicate in SV40LT-immortalized mouse hepatic cells. The expressions of hepatitis B surface antigen and hepatitis B c antigen, as well as the presence of HBV DNA replication intermediates, were observed in the pHBV1.3-transfected cells. This cell model will contribute to the research of HBV and the evaluation of anti-viral drugs in vivo.