Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection

Figure 1 Real time polymerase chain reaction standard curve and the linear relationship between Campylobacter jejuni quantity and polymerase chain reaction cycle threshold. A: The standard curve was constructed using genomic DNA from Campylobacter jejuni isolates. Serial dilutions ranging from 100 to 106 CFU/mL of target template were subjected to real time polymerase chain reaction assay; B: The linear relationship between Log CFU/mL and threshold cycles. RFU: Relative fluorescence units.

Figure 2 Stability tests. A: Curves constructed based dilutions of the genomic DNA of Campylobacter jejuni (C. jejuni) NCTC11168; B: Curves constructed based on the dilution of the genomic DNA of C. jejuni ATCC33560; C: Curves generated from the serial dilution of the genomic DNA of C. jejuni 81116, ATCC49349 and ICDCCJ07001. Ct: Threshold cycle.