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World J Gastroenterol. May 28, 2013; 19(20): 3090-3095
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3090
Published online May 28, 2013. doi: 10.3748/wjg.v19.i20.3090
Development and application of a real-time polymerase chain reaction method for Campylobacter jejuni detection
Mao-Jun Zhang, Bo Qiao, Jian-Zhong Zhang, State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
Xue-Bin Xu, Shanghai Municipal Center for Disease Control and Prevention, Shanghai 200336, China
Author contributions: Zhang MJ designed the research, analyzed the data and wrote the paper; Qiao B performed the research and analyzed the data; Xu XB collected the clinical samples and analyzed the data; Zhang JZ analyzed the data.
Supported by The General Program of National Natural Science Foundation of China, No. 81271789; and the Major State Basic Research Development Program, No. 2013CB127204
Correspondence to: Dr. Mao-Jun Zhang, State Key Laboratory for Infectious Disease Prevention and Control, and National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping District, Beijing 102206, China. zhangmaojun@icdc.cn
Telephone: +86-10-58900755 Fax: +86-10-58900700
Received: January 15, 2013
Revised: March 7, 2013
Accepted: April 9, 2013
Published online: May 28, 2013
Processing time: 133 Days and 11.7 Hours
Revised: March 7, 2013
Accepted: April 9, 2013
Published online: May 28, 2013
Processing time: 133 Days and 11.7 Hours
Core Tip
Core tip: In the present study, we developed an effective real-time polymerase chain reaction method based on the specific DNA sequence of the hipO gene in Campylobacter jejuni (C. jejuni). The detection limit of this assay is 4.3 CFU/mL. A study of 242 clinical stool specimens from diarrheal patients indicated that this method is more sensitive than direct bacterial culture for the identification of C. jejuni from stool specimens.